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Abstract <p>In the context of growing environmental challenges and the need for efficient waste utilization, the conversion of waste cooking oils (WCO) into biodegradable materials represents a promising direction in modern biotechnology. This study evaluates the suitability of sunflower-based WCO, collected from public catering facilities in Saint Petersburg, as a substrate for the biosynthesis of polyhydroxyalkanoates (PHA) using the strain <i>Cupriavidus necator</i> H16. A comparative assessment was carried out to determine the productivity when using refined, unrefined, and waste sunflower oils. The highest PHA yield was achieved with WCO (13.70 ± 1.95 g/L, 26.80%), which is comparable to the result obtained with unrefined oil (13.20 ± 0.87 g/L, 20.38%) and exceeds the yield observed on glucose (11.43 ± 0.76 g/L, 25.88%). Despite the complex and heterogeneous nature of WCO, <i>C. necator</i> H16 demonstrated a high degree of adaptation to different lipid-based substrates. The findings highlight the potential of WCO as an alternative carbon source within the circular economy framework and provide a basis for further optimization of PHA biosynthesis from food industry residues.</p>

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Utilization of Waste-Derived Fatty Acid Feedstock for Polyhydroxyalkanoate Biosynthesis by Cupriavidus necator H16

  • 期刊论文

  • 发表日期：

    2025年5月17日

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  • А. P. Kuznetsova,
  • М. E. Lysenko,
  • R. I. Al-Shekhadat

摘要

Abstract

In the context of growing environmental challenges and the need for efficient waste utilization, the conversion of waste cooking oils (WCO) into biodegradable materials represents a promising direction in modern biotechnology. This study evaluates the suitability of sunflower-based WCO, collected from public catering facilities in Saint Petersburg, as a substrate for the biosynthesis of polyhydroxyalkanoates (PHA) using the strain Cupriavidus necator H16. A comparative assessment was carried out to determine the productivity when using refined, unrefined, and waste sunflower oils. The highest PHA yield was achieved with WCO (13.70 ± 1.95 g/L, 26.80%), which is comparable to the result obtained with unrefined oil (13.20 ± 0.87 g/L, 20.38%) and exceeds the yield observed on glucose (11.43 ± 0.76 g/L, 25.88%). Despite the complex and heterogeneous nature of WCO, C. necator H16 demonstrated a high degree of adaptation to different lipid-based substrates. The findings highlight the potential of WCO as an alternative carbon source within the circular economy framework and provide a basis for further optimization of PHA biosynthesis from food industry residues.