Synthesis of Some New Amides Containing Heterocyclic Units Based on Hydrazide Derivatives

A series of novel amide compounds with 1,2,4-triazine, 1,2,4-triazole and 1,3,4-oxadiazole moieties were synthesized by multistep procedure from 1,2,4-triazine-containing hydrazine-carbothioamides. Numerous synthetic compounds were tested for their ability to inhibit the growth of both gram-positive (Bacillus subtitis and Staphylococcus aureus) and gram-negative (E. coli) bacteria. All the tested compounds had no effects on fungi (Candida tropicalis). The toxicity of the synthesized chemicals was checked to guarantee their safety. The enzymic activity was studied, the enzymatic activity was not affected by the compounds under examined, and the levels of uric acid and creatine did not exceed normal limits.