Simultaneous Investigation of Dopamine and Uric Acid Using Novel Electrochemical Sensor Based on Green Synthesized Silver Vanadate Nanoparticles

A novel, highly sensitive, and precise electrochemical biosensor designed for the simultaneous determination of dopamine (DA) and uric acid (UA) via cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods by using silver vanadate nanoparticles (AgVO₃ NPs) fabricated glassy carbon electrode (GCE). AgVO₃ NPs were prepared by eco-friendly simple green route approach by using Samanea saman (rain tree) tree pod extract. The structural morphology and characterization of as-prepared AgVO₃ NPs were examined using powder XRD, FT-IR, FESEM, elemental mapping, EDAX, and HR-TEM analysis. Using DPV DA, and UA were simultaneously determined at AgVO₃ NPs modified GCE under optimal conditions. The biosensor’s low limits of detection for DA and UA were 0.019 and 0.012 µM, respectively. The developed sensor could be used as a platform for routine research into DA and UA because of its outstanding reproducibility and stability. The developed sensor was also employed for the analysis of DA and UA in serum and urine samples with excellent recovery.