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Abstract <p>The interaction of serotonin with DNA has been studied by methods of fluorescence and absorption spectroscopies. Serotonin fluorescence intensity was shown to decrease (quench) with increasing concentrations of DNA in the solution. Based on the intensity measurements, the values of the quenching constants (Stern–Volmer constant)<i>—K</i><sub>SV</sub> were determined in the temperature interval 298–311 K. It was also shown that the absorption spectra at increasing concentrations of serotonin showed a higher degree in the absence of DNA, as compared to the presence of DNA. Based on the fluorescence and absorption spectra of the serotonin complexes with DNA, the adsorption isotherms were obtained, and the values of the binding constant <i>K</i> and the number of base pairs per binding site <i>n</i> were determined.</p>

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To the Mechanisms of Interaction of the Neurotransmitter Serotonin with DNA

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • M. A. Parsadanyan,
  • A. P. Antonyan,
  • M. A. Shahinyan,
  • A. V. Vardanyan,
  • G. H. Kocharyan,
  • M. N. Gharibyan,
  • P. O. Vardevanyan

摘要

Abstract

The interaction of serotonin with DNA has been studied by methods of fluorescence and absorption spectroscopies. Serotonin fluorescence intensity was shown to decrease (quench) with increasing concentrations of DNA in the solution. Based on the intensity measurements, the values of the quenching constants (Stern–Volmer constant)—KSV were determined in the temperature interval 298–311 K. It was also shown that the absorption spectra at increasing concentrations of serotonin showed a higher degree in the absence of DNA, as compared to the presence of DNA. Based on the fluorescence and absorption spectra of the serotonin complexes with DNA, the adsorption isotherms were obtained, and the values of the binding constant K and the number of base pairs per binding site n were determined.