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Abstract <p><b>Objective:</b> The <i>Hymenobacter psoromatis</i> (formerly Hymenobacter sp. PAMC 26554) rhodopsin HbR1 gene was identified <i>via</i> bioinformatic analysis. <i>H. psoromatis</i> is a UV-resistant bacterium, the representative of the genus <i>Hymenobacter,</i> widespread in the cryosphere. The <i>Hymenobacter</i> genus microorganisms are Gram-negative, aerobic, rod-shaped, they have red-pink pigmentation, due to the presence of carotenoids. The bacterial pigmentation serves for UV-resistance, photo-protection, lightharvesting probably in the connection with the microbial rhodopsins. The specific biological role of the HbR1 within its host remains unknown but rhodopsin has some unusual properties observed experimentally. <b>Methods:</b> We performed a multidisciplinary study which involves both experimental and theoretical methods to characterize spectroscopic properties of <i>Hymenobacter psoromatis</i> rhodopsin HbR1. <b>Results and Discussion:</b> The predicted structure of HbR1 has low similarity with the structures of the main groups of known rhodopsins. QM/MM-calculated absorption spectra show a good agreement with experimental data. <b>Conclusions:</b> Our results demonstrate a good agreement between experimental and theoretical data. Based on our study, we provide a spectroscopic characterization of HbR1 and propose a possible mechanism for spectral tuning of this protein.</p>

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Spectral Characterisation of Hymenobacter psoromatis Rhodopsin: The Theoretical and Experimental Study

  • 期刊论文

  • 发表日期：

    2026年7月28日

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  • A. Kh. Taldaev,
  • A. V. Pavlov,
  • A. S. Limonova,
  • D. V. Smutin,
  • D. A. Fedotov,
  • I. S. Okhrimenko

摘要

Abstract

Objective: The Hymenobacter psoromatis (formerly Hymenobacter sp. PAMC 26554) rhodopsin HbR1 gene was identified via bioinformatic analysis. H. psoromatis is a UV-resistant bacterium, the representative of the genus Hymenobacter, widespread in the cryosphere. The Hymenobacter genus microorganisms are Gram-negative, aerobic, rod-shaped, they have red-pink pigmentation, due to the presence of carotenoids. The bacterial pigmentation serves for UV-resistance, photo-protection, lightharvesting probably in the connection with the microbial rhodopsins. The specific biological role of the HbR1 within its host remains unknown but rhodopsin has some unusual properties observed experimentally. Methods: We performed a multidisciplinary study which involves both experimental and theoretical methods to characterize spectroscopic properties of Hymenobacter psoromatis rhodopsin HbR1. Results and Discussion: The predicted structure of HbR1 has low similarity with the structures of the main groups of known rhodopsins. QM/MM-calculated absorption spectra show a good agreement with experimental data. Conclusions: Our results demonstrate a good agreement between experimental and theoretical data. Based on our study, we provide a spectroscopic characterization of HbR1 and propose a possible mechanism for spectral tuning of this protein.