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Abstract <p><b>Objective:</b> The development of drought-tolerant genotypes of <i>Quercus robur</i> (“English” oak) is an important task in the context of climate change. This study aimed to analyze the transcriptomes of two oak genotypes with contrasting drought tolerance under normal water availability and water deficit conditions. <b>Methods:</b> Transcriptome samples were collected from two oak genotypes differing in drought tolerance. The study was conducted under two conditions: normal water availability (control) and water deficit (drought). Differentially expressed genes (DEGs) were identified to compare the responses between the two genotypes under both conditions. <b>Results and Discussion:</b> In genotype 1 (drought-tolerant), 70 DEGs were identified, with 42 upregulated and 28 downregulated. In genotype 2 (drought-sensitive), 97 DEGs were identified, with 37 upregulated and 60 downregulated. Genotype 1 exhibited a strong overrepresentation of GO terms related to abiotic stress response and changes in photosynthesis, suggesting a better adaptation to drought conditions. Genotype 2 showed DEGs primarily related to stress-induced regulation of hormone biosynthesis and stress response in the extracellular matrix, indicating a weaker drought response. An overlap analysis revealed one common gene with increased expression in both genotypes under drought: methylsterol monooxygenase, involved in steroid biosynthesis. <b>Conclusions:</b> The results provide insights into the molecular mechanisms behind drought tolerance in <i>Quercus robur</i>. The identification of differentially expressed genes could help in early identification of drought-tolerant genotypes, aiding in the development of oak varieties more suitable for future climate conditions.</p>

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Comparative Transcriptome Analysis of Two Related but Contrasting Quercus robur Genotypes under Drought Stress

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • T. S. Tikhomirova,
  • V. G. Lebedev,
  • E. O. Vidyagina,
  • A. A. Popova,
  • P. M. Evlakov,
  • T. A. Grodetskaya,
  • I. M. Nartova,
  • V. S. Ryzhkova,
  • K. A. Shestibratov

摘要

Abstract

Objective: The development of drought-tolerant genotypes of Quercus robur (“English” oak) is an important task in the context of climate change. This study aimed to analyze the transcriptomes of two oak genotypes with contrasting drought tolerance under normal water availability and water deficit conditions. Methods: Transcriptome samples were collected from two oak genotypes differing in drought tolerance. The study was conducted under two conditions: normal water availability (control) and water deficit (drought). Differentially expressed genes (DEGs) were identified to compare the responses between the two genotypes under both conditions. Results and Discussion: In genotype 1 (drought-tolerant), 70 DEGs were identified, with 42 upregulated and 28 downregulated. In genotype 2 (drought-sensitive), 97 DEGs were identified, with 37 upregulated and 60 downregulated. Genotype 1 exhibited a strong overrepresentation of GO terms related to abiotic stress response and changes in photosynthesis, suggesting a better adaptation to drought conditions. Genotype 2 showed DEGs primarily related to stress-induced regulation of hormone biosynthesis and stress response in the extracellular matrix, indicating a weaker drought response. An overlap analysis revealed one common gene with increased expression in both genotypes under drought: methylsterol monooxygenase, involved in steroid biosynthesis. Conclusions: The results provide insights into the molecular mechanisms behind drought tolerance in Quercus robur. The identification of differentially expressed genes could help in early identification of drought-tolerant genotypes, aiding in the development of oak varieties more suitable for future climate conditions.