Finger-Actuated Microfluidic Platform for Colorimetric Isothermal Diagnostics of Neisseria meningitidis and Herpes Simplex Virus

Objective: The 2020 pandemic showed a need for quick, accessible, and user-friendly diagnostic tests to detect various pathogens applicable at the point-of-care (POC) or at home. Such diagnostics should require a minimum of steps and equipment. Results and Discussion: In this study, we have manufactured and modified a one-way valve system for the microfluidic platform and integrate it to the device for DNA extraction and loop-mediated isothermal amplification (LAMP) with a color readout which allowed rapid and effective diagnosis of Neisseria meningitidis (N. meningitidis) and herpes simplex virus (HSV) without the use of expensive equipment. The article describes the aspects of the manufacturing process and a possible presentation of the platform. Conclusions: This article presents a solution of integrating of embedded flexible films with ion-dependent colorimetric visualization.