Antioxidant and Antibiofilm Activity of Laccase-Mediated Oxidized Products of Ferulic Acid

Objective: The enzymatic oxidation of ferulic acid was carried out to improve their solubility and stability and provide new properties. Methods: The oxidation of ferulic acid was carried out using laccase from Trametes versicolor in sodium acetate buffer of pH 5.0 at room temperature. The two oxidized products were fractionated using column chromatography. These oxidized products, P1 and P2, demonstrated good antibiofilm and antioxidant activity compared to ferulic acid. P1 exhibited higher antibiofilm and antioxidant activity than P2. These products were characterized using FT-IR and NMR spectroscopies. Results and Discussion: A polymeric form of ferulic acid was synthesized using the enzyme laccase and fractionated using two different solvents. These fractions were purified, dried, and evaluated for antibiofilm and antioxidant potential. P1 and P2 have MIC of 125 µg against S. aureus. Sub-MIC concentrations were used for antibiofilm assays. The results suggested that P1 and P2 have good antibiofilm activity than ferulic acid. Conclusions: The predicted structure of P1 was a dimer, and P2 was synthesized as a trimer. These findings suggested that, when compared to the trimer, the dimer product of ferulic acid oxidation has good antibiofilm and antioxidant activity.