Comparison of Field and Laboratory Testing of Copper Phytotoxicity in Industrially Polluted Soils
摘要
Phytotoxicity assessment of metals in soils is typically conducted through laboratory testing. This study compares the field and laboratory evaluations of copper phytotoxicity in soils contaminated by copper mining and examines how test duration influences the observed toxicity. Barley shoots (Hordeum vulgare L.) and rhizospheric soils were sampled along a visible gradient of copper contamination in the Kargaly mining district (Orenburg Region, Russia). The soils, classified as chernozem (Mollisol), contained up to 7000 mg/kg of total copper, while concentrations of other elements remained at background levels, indicating a distinct monometallic contamination. Field experiments showed no statistically significant difference in the effective concentration causing a 50% reduction in plant response (EC50) between short-term (40 days) and long-term (97 days) exposure. Laboratory phytotests followed ISO 11 269-2 (21 days) and ISO 22 030 (60 days) protocols. Short-term (21-day) tests revealed no significant relationship between soil copper content and barley growth or shoot copper accumulation. In contrast, 60-day tests demonstrated an inverse relationship between total soil copper and both plant growth and shoot copper concentration. However, it should be noted that substantial differences in EC50 values were detected between the long-term laboratory experiment and the long-term field experiment. This discrepancy highlights the need for further research to identify laboratory phytotesting conditions that more accurately reflect metal toxicity to plants under realistic field and agricultural conditions.