Development of Immunoradiometric Assay Technique for Measurement of Luteinizing Hormone in Human Serum

This study was aimed to prepare immunoradiometric (IRMA) system using solid-phase magnetizable cellulose particles coupled with luteinizing hormone (LH) to determine LH in human serum. The LH-IRMA system was designed through preparation of LH polyclonal antibody (anti-LH), ¹²⁵I-LH tracer, and LH standards. Production of anti-LH was carried out through immunization of three Balb/C mice with LH-BSA immunogen. Magnetizable cellulose particles were activated using 1,1’-carbonyldiimidazole (CDI) and coupled with purified anti-LH. Optimization and validation of LH-IRMA were undertaken. The results obtained show that the local solid-phase magnetic particle LH-IRMA is a sensitive, specific, precise, and accurate method for measuring the LH concentration in human serum and can be used as a diagnostic tool for some disorders such as infertility in females and impotence in males.