Abstract <p>As a result of the research performed, the emission of CO₂ from soils in the southern tundra ecosystems of the northeastern Russian Plain has been estimated using the example of the environs of Vorkuta. The soil cover of the studied area is presented by Histic Turbic Cryosol, Histic Reductaquic Glacic Cryosol, Reductaquic Glacic Cryosol, and Reductaquic Glacic Cryosol. Atypically high values of CO₂ emission from soils [2.13 ± 0.13 g C/(m² day)] were largely due to the weather of the 2022 growing season: high air temperatures and low precipitation. About 60% of the variability in the emission value was due to the content of microbial biomass carbon and extractable soil carbon, temperature, and soil moisture. High spatial variation in the content of extractable carbon and microbial biomass carbon and parameters of hydrothermal regime of soils was found. The soils were characterized by low values of extractable organic carbon and soil microbial biomass carbon (224 ± 18 and 873 ± 73 mg C/kg of soil, respectively). The thickness of organic horizon of soil determines 72% of variability in the content of microbial biomass carbon and 79% of variability in the content of extractable carbon. Regular measurements of CO₂ emissions from soils of tundra ecosystems in the northeast of the Russian Plain should obtain special attention, as this will improve the accuracy of assessing the global greenhouse gas flows.</p>