Complex Study of the Internal Structure of Polymeric Sponge Matrixes during Tissue-Engineered Arrangement
摘要
Abstract
The possibilities of a complex study of the 3D structure of a polymeric spongy matrix based on polylactide, using X-ray microtomography without staining in combination with noninvasive method scanning impulse acoustic microscopy and energy-dispersive X-ray microanalysis, are presented. Comparative studies of pure matrix samples, in vitro samples kept in a culture medium and after their implantation in vivo were carried out. The use of a complex of noninvasive methods revealed changes in the microstructure, including matrix degradation processes in a culture medium, after incubation with cells and implantation in a laboratory animal.