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Monitoring the Formation of Microcrystal Suspensions of Proteins Used in Serial Microcrystallography by Small-Angle X-ray Scattering

  • P. V. Konarev,
  • G. S. Peters,
  • V. R. Samygina

摘要

Abstract

Serial crystallography, which has been extensively developed in the past years, makes it possible to study dynamic processes occurring in protein molecules. The method is implemented at fourth-generation synchrotron sources or free electron lasers and it requires suspensions of microcrystals. It was demonstrated that small-angle X-ray scattering can be used to monitor the formation of such suspensions in crystallization conditions as exemplified by lysozyme.