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Abstract— <p>As part of the Biorisk space experiment, containers containing a dry sample of Lambda phage DNA were exposed on the outer surface of the International Space Station for a long time (1st session, 815 (of which 656 were on the outer surface) days and 2nd session, 1179 (of which 961 were on the outer surface) days). After returning to Earth, the samples were dissolved and subjected to the action of restrictases (BsuR I and Sse9 I), followed by electrophoresis in agarose gel. With increasing exposure time and, accordingly, the absorbed dose (from 614 mGy in the 1st session to 750 mGy in the 2nd session), differences in the size and number of fragments for flight and control samples increase.</p>

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Fragmentation of Lambda Phage DNA under the Influence of Cosmic Radiation during Prolonged Exposure in Containers on the Outer Surface of the International Space Station

  • 期刊论文

  • 发表日期：

    2026年4月1日

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  • I. B. Alchinova,
  • M. V. Polyakova,
  • S. A. Kharin,
  • M. Yu. Karganov

摘要

Abstract—

As part of the Biorisk space experiment, containers containing a dry sample of Lambda phage DNA were exposed on the outer surface of the International Space Station for a long time (1st session, 815 (of which 656 were on the outer surface) days and 2nd session, 1179 (of which 961 were on the outer surface) days). After returning to Earth, the samples were dissolved and subjected to the action of restrictases (BsuR I and Sse9 I), followed by electrophoresis in agarose gel. With increasing exposure time and, accordingly, the absorbed dose (from 614 mGy in the 1st session to 750 mGy in the 2nd session), differences in the size and number of fragments for flight and control samples increase.