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Abstract <p>Synthetic glycolipids and similar amphiphils with peptide and other head groups have been designed for labeling/modification of living cells under mild physiological conditions. Under-standing the mechanism of their penetration through the cellular glycocalyx and subsequent insertion into the plasma membrane opens up the prospect of improving the recently found anti-tumor properties of such constructs. In this work, we applied small-angle X-ray scattering (SAXS) technique to characterize structure of nanoparticles formed by self-assembly of synthetic glycolipid A (type 2)-Ad-DE and to estimate its dependence on the glycolipid concentration. The studies were performed at a range of SAXS-applicable concentrations. The obtained results indicate that self-assembly process leads to formation of monodisperse nanoparticles with micelle-like architecture, which is maintained regardless of concentration, indicating absence of the nanoparticle’s positive interaction with their glycopart. We applied <i>ab initio</i> modeling that showed a good agreement with experimental data, and found that the ellipsoid monodisperse nanoparticles have a size of about 14 nm. Quasi-atomic modeling visualised that glycan ligands are well accessed for biological recognition. This knowledge will facilitate further study of the formation of the supramolecular form(s) of A(type 2)-Ad-DE and other glycolipids within the glycocalyx and its further fate in new therapeutic strategies.</p>

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Structure of Supramers Formed by Glycolipid Analogues: SAXS Study

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • I. S. Vaskan,
  • M. V. Petoukhov,
  • N. V. Bovin,
  • I. M. Ryzhov,
  • V. A. Dimitreva,
  • E. V. Shtykova,
  • V. A. Oleinikov,
  • A. V. Zalygin

摘要

Abstract

Synthetic glycolipids and similar amphiphils with peptide and other head groups have been designed for labeling/modification of living cells under mild physiological conditions. Under-standing the mechanism of their penetration through the cellular glycocalyx and subsequent insertion into the plasma membrane opens up the prospect of improving the recently found anti-tumor properties of such constructs. In this work, we applied small-angle X-ray scattering (SAXS) technique to characterize structure of nanoparticles formed by self-assembly of synthetic glycolipid A (type 2)-Ad-DE and to estimate its dependence on the glycolipid concentration. The studies were performed at a range of SAXS-applicable concentrations. The obtained results indicate that self-assembly process leads to formation of monodisperse nanoparticles with micelle-like architecture, which is maintained regardless of concentration, indicating absence of the nanoparticle’s positive interaction with their glycopart. We applied ab initio modeling that showed a good agreement with experimental data, and found that the ellipsoid monodisperse nanoparticles have a size of about 14 nm. Quasi-atomic modeling visualised that glycan ligands are well accessed for biological recognition. This knowledge will facilitate further study of the formation of the supramolecular form(s) of A(type 2)-Ad-DE and other glycolipids within the glycocalyx and its further fate in new therapeutic strategies.