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Abstract <p>Several versions of gold nanoparticles are proposed for detecting formazan formed by the enzymatic reduction of MTT reagent by <i>E. coli</i> enzymes. It is shown that gold nanostars coated with a micellar stabilizer are the most promising SERS substrates for detecting formazan in biological mixtures, reducing the required titer of bacteria by at least an order of magnitude.</p>

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Gold Nanoparticles as SERS Substrates for MTT Assays

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • V. A. Mushenkov,
  • A. M. Burov,
  • V. I. Kukushkin,
  • E. G. Zavyalova

摘要

Abstract

Several versions of gold nanoparticles are proposed for detecting formazan formed by the enzymatic reduction of MTT reagent by E. coli enzymes. It is shown that gold nanostars coated with a micellar stabilizer are the most promising SERS substrates for detecting formazan in biological mixtures, reducing the required titer of bacteria by at least an order of magnitude.