Abstract <p>Because of the need to expand the range of promising Russian-manufacture reagents to replace import analogs, the authors study effect exerted by reagents from class of dithiocarbamates on the surface of sulfide minerals in composition of complex gold ore. New research methods are used to examine interaction of the test reagents with the gold-carrying minerals in flotation of complex ore. It is found that a new reagent of bis-piperazine dithiocarbamate is an efficient selective collector of mineral carriers of noble metals in flotation of complex gold ore. The test methods were UV spectroscopy, wetting angle measurement, scanning electron microscopy and laser scanning microscopy. Different adsorbed phases of the reagent on the polished section are determined and visualized. Selective attachment of the reagent at ore minerals can ensure their selective extraction in different concentrates in flotation of complex ore.</p>