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<p>The study was conducted to evaluate the expression patterns of genes regulating myogenesis (<i>Myf5</i>, <i>MyoG</i>, <i>MyoD1a</i>, <i>MyoD1b</i>, <i>MyoD1c</i>, <i>MSTN1a</i> and <i>MSTN1b</i>, <i>MyHC</i>), as well as growth in underyearlings (0+) Atlantic salmon kept under different lighting regimes. The results obtained for fish from groups with experimental lighting regimes—continuous (24L:0D) and 12L:12D, were compared with those for underyearlings from the control group (standard lighting conditions of the fish hatchery without additional sources of artificial light) and the group with a regime simulating natural lighting regime typical for the Belomorsky region (NatL:D). The experiment was carried out in the fish hatchery with natural seasonal fluctuations in water temperature. It was found that fish kept under continuous lighting had a higher body weight throughout the experiment, in contrast to individuals from other groups. Underyearlings salmon from the group with the 12L:12D regime were the smallest in weight. The expression levels of the genes <i>MyHC</i>, <i>MyoG</i>, <i>MyoD1a</i>, <i>MyoD1b</i> and <i>MyoD1c</i> were higher in fish from groups with artificial lighting (24L:0D, 12L:12D, NatL:D) compared to those in salmon individuals in the control group. The identified intergroup differences in the simultaneous expression of the studied genes indicate an intensification of the proliferation and differentiation processes of muscle cells in fish from the experimental groups in response to the action of additional lighting. Differential expression of <i>MyoD1</i> gene paralogs throughout the experimental period was shown. The expression level of the <i>MyoD1c</i> gene increased by the end of the experiment together with the levels of <i>MyoG</i> and <i>MyHC</i>, while <i>MyoD1b</i> decreased along with the expression of <i>Myf5</i>. Changes in the expression levels of the above genes were accompanied by an increase in the expression of the myostatin gene paralogues <i>MSTN1a</i> and <i>MSTN1b</i>. Differences in the expression patterns of myogenic regulatory factors between groups with different lighting regimes indicate that the regulation of myogenesis in fish depends on the length of the photoperiod.</p>

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Expression of Genes Regulating Muscle Growth in Atlantic Salmon Underyearlings Grown under Different Lighting Regimes

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • N. S. Shulgina,
  • M. V. Kuznetsova,
  • N. N. Nemova

摘要

The study was conducted to evaluate the expression patterns of genes regulating myogenesis (Myf5, MyoG, MyoD1a, MyoD1b, MyoD1c, MSTN1a and MSTN1b, MyHC), as well as growth in underyearlings (0+) Atlantic salmon kept under different lighting regimes. The results obtained for fish from groups with experimental lighting regimes—continuous (24L:0D) and 12L:12D, were compared with those for underyearlings from the control group (standard lighting conditions of the fish hatchery without additional sources of artificial light) and the group with a regime simulating natural lighting regime typical for the Belomorsky region (NatL:D). The experiment was carried out in the fish hatchery with natural seasonal fluctuations in water temperature. It was found that fish kept under continuous lighting had a higher body weight throughout the experiment, in contrast to individuals from other groups. Underyearlings salmon from the group with the 12L:12D regime were the smallest in weight. The expression levels of the genes MyHC, MyoG, MyoD1a, MyoD1b and MyoD1c were higher in fish from groups with artificial lighting (24L:0D, 12L:12D, NatL:D) compared to those in salmon individuals in the control group. The identified intergroup differences in the simultaneous expression of the studied genes indicate an intensification of the proliferation and differentiation processes of muscle cells in fish from the experimental groups in response to the action of additional lighting. Differential expression of MyoD1 gene paralogs throughout the experimental period was shown. The expression level of the MyoD1c gene increased by the end of the experiment together with the levels of MyoG and MyHC, while MyoD1b decreased along with the expression of Myf5. Changes in the expression levels of the above genes were accompanied by an increase in the expression of the myostatin gene paralogues MSTN1a and MSTN1b. Differences in the expression patterns of myogenic regulatory factors between groups with different lighting regimes indicate that the regulation of myogenesis in fish depends on the length of the photoperiod.