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Abstract <p>This experiment, including greenhouse and laboratory experiments, was carried out by the faculty of plant breeding at Mohaghegh Ardabili University, containing forty-five promising lines of durum wheat in 2018–2019. The factorial experimental design was in the complete blocks form with three replications. The result of the variance analysis of LT50 showed a significant difference between the genotypes at the 1 percent. The mean comparison by the LSD test method showed varied LT50 between –0.754 and –26.609 values. Lines 34 and 37 were identified as resistant lines in the mean LT50. Genotypes are divided into five groups in clustering based on the LT50. 117 bands of ISSR primer were created, of which 93 bands were polymorphic and 24 bands were monomorphic. The PIC, the MI, the EMR, the RP, the Nei, and the Shannon indexes were measured. General cluster analysis was performed with all genotypes to determine the relationship between the studied genotypes. Dice genetic similarity distance and the UPGMA method were selected based on the highest cophenetic correlation coefficient. The studied lines were divided into five groups. Stepwise regression analysis was performed to evaluate the relationship between measured traits and molecular markers. All traits in the four stress levels showed a significant correlation with some ISSR markers. The LT50 was correlated with nine markers; the highest positive correlation was with marker 5d, and the highest negative correlation was with marker 2i.</p>

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Genetic Diversity Analysis of Advanced Durum Wheat Lines Using ISSR Markers

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • Fatemeh Mohammadi-Azar,
  • Omid Sofalian,
  • Ali Asghari,
  • Asghar Ebadi,
  • Rahmatollah Karimizadeh,
  • Sajjad Moharramnejad,
  • Negin Taleschian Tabrizi

摘要

Abstract

This experiment, including greenhouse and laboratory experiments, was carried out by the faculty of plant breeding at Mohaghegh Ardabili University, containing forty-five promising lines of durum wheat in 2018–2019. The factorial experimental design was in the complete blocks form with three replications. The result of the variance analysis of LT50 showed a significant difference between the genotypes at the 1 percent. The mean comparison by the LSD test method showed varied LT50 between –0.754 and –26.609 values. Lines 34 and 37 were identified as resistant lines in the mean LT50. Genotypes are divided into five groups in clustering based on the LT50. 117 bands of ISSR primer were created, of which 93 bands were polymorphic and 24 bands were monomorphic. The PIC, the MI, the EMR, the RP, the Nei, and the Shannon indexes were measured. General cluster analysis was performed with all genotypes to determine the relationship between the studied genotypes. Dice genetic similarity distance and the UPGMA method were selected based on the highest cophenetic correlation coefficient. The studied lines were divided into five groups. Stepwise regression analysis was performed to evaluate the relationship between measured traits and molecular markers. All traits in the four stress levels showed a significant correlation with some ISSR markers. The LT50 was correlated with nine markers; the highest positive correlation was with marker 5d, and the highest negative correlation was with marker 2i.