Scholarlink

Abstract <p>The recent advancements in small RNA high-throughput sequencing have vastly enhanced our understanding of the small RNA repertoire, and it has been revealed that some housekeeping non-coding RNAs, including tRNA and rRNA, act as sources of small RNAs. In this study, we re-analyzed publicly accessible PANDORA-seq data to uncover 28s5-rtsRNA, the small RNA originating from the first nucleotide at the 5' terminus of 28s rRNA, as the most prevalent small RNA class across multiple tissues. Furthermore, we discovered a novel category termed ‘28s5-rtsRNA half,’ which is highly expressed in sperm. When paired with 5.8s rRNA 3' terminus-derived small RNA or complementary single-stranded RNA, 28s5-rtsRNA duplexes elevate the 28s/18s rRNA ratios and decrease the mRNA levels of several ribosomal proteins, whereas single-stranded 28s5-rtsRNA alone lacks these effects. Mutational scanning analysis pinpointed the first seven nucleotides of 28s5-rtsRNA as essential for its biological functions. Our findings highlight the abundance of 28s5-rtsRNA across tissues and its potential role in regulating ribosomal protein mRNA levels, paving the way for future studies to comprehensively elucidate the mechanisms and functions of 28s5-rtsRNA in diverse biological processes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The Profile and Biological Functions of 28s rRNA 5' Terminus-Derived Small RNA

  • 期刊论文

  • 发表日期：

    2025年12月1日

查看全文
  • Weijun Su,
  • Yi Zhang,
  • Ziran Wang,
  • Yi Wang,
  • Yanjie Zhao,
  • Shuai Li

摘要

Abstract

The recent advancements in small RNA high-throughput sequencing have vastly enhanced our understanding of the small RNA repertoire, and it has been revealed that some housekeeping non-coding RNAs, including tRNA and rRNA, act as sources of small RNAs. In this study, we re-analyzed publicly accessible PANDORA-seq data to uncover 28s5-rtsRNA, the small RNA originating from the first nucleotide at the 5' terminus of 28s rRNA, as the most prevalent small RNA class across multiple tissues. Furthermore, we discovered a novel category termed ‘28s5-rtsRNA half,’ which is highly expressed in sperm. When paired with 5.8s rRNA 3' terminus-derived small RNA or complementary single-stranded RNA, 28s5-rtsRNA duplexes elevate the 28s/18s rRNA ratios and decrease the mRNA levels of several ribosomal proteins, whereas single-stranded 28s5-rtsRNA alone lacks these effects. Mutational scanning analysis pinpointed the first seven nucleotides of 28s5-rtsRNA as essential for its biological functions. Our findings highlight the abundance of 28s5-rtsRNA across tissues and its potential role in regulating ribosomal protein mRNA levels, paving the way for future studies to comprehensively elucidate the mechanisms and functions of 28s5-rtsRNA in diverse biological processes.