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Abstract <p>PP2C (Protein Phosphatase 2C) is the largest family of protein phosphatase in plants, which participates in the regulation of plant growth, development, and stress responses through dephosphorylation. The number of its members varies among different plant species, highlighting its significant role in plant evolution and adaptation. In this study, a <i>PP2C</i> gene was cloned from <i>Salvia miltiorrhiza</i>, with the coding sequences (CDS) of 1161 base pairs, encoding a protein containing 386 amino acids. Bioinformatics analysis revealed it is a member of the PP2C family B group, with the highest homology to AtPP2C14. Expression analysis results showed that <i>SmPP2C14</i> is highly expressed in flowers, and influenced by plant hormones (SA and IAA) and drought stress. Subcellular localization indicated that SmPP2C14 is exclusively located in the nucleus. The <i>SmPP2C14</i> gene may play an important role in the stress response and the accumulation of secondary metabolites in <i>S. miltiorrhiza</i>. The study of <i>SmPP2C14</i> is of great significance for the future development of plant varieties with strong stress resistance, high quality, and high yield.</p>

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Cloning, Expression Analysis and Subcellular Localization of PP2C14 in Salvia miltiorrhiza

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • Yongfeng Xie,
  • Hao Liu

摘要

Abstract

PP2C (Protein Phosphatase 2C) is the largest family of protein phosphatase in plants, which participates in the regulation of plant growth, development, and stress responses through dephosphorylation. The number of its members varies among different plant species, highlighting its significant role in plant evolution and adaptation. In this study, a PP2C gene was cloned from Salvia miltiorrhiza, with the coding sequences (CDS) of 1161 base pairs, encoding a protein containing 386 amino acids. Bioinformatics analysis revealed it is a member of the PP2C family B group, with the highest homology to AtPP2C14. Expression analysis results showed that SmPP2C14 is highly expressed in flowers, and influenced by plant hormones (SA and IAA) and drought stress. Subcellular localization indicated that SmPP2C14 is exclusively located in the nucleus. The SmPP2C14 gene may play an important role in the stress response and the accumulation of secondary metabolites in S. miltiorrhiza. The study of SmPP2C14 is of great significance for the future development of plant varieties with strong stress resistance, high quality, and high yield.