Scholarlink

Abstract <p>Lactic acid bacteria (LAB) are ubiquitous probiotics which provide health benefits to the host when consumed in optimal concentrations. <i>Lactobacillus</i> spp<i>.</i>, an essential member of the Lactic acid bacteria (LAB), are widely recognized as safe. An indigenous probiotic of the same ecological niche can be more effective due to its environmental compatibility and high adaptability. This study aims to assess the probiotic potential and genetic diversity of an indigenous strain CF-7, isolated from a domestically reared Sahiwal calf by phenotypic and whole genome analysis. Strain CF-7 exhibited desirable probiotic traits, including gastrointestinal tolerance (85.6–95.1%) auto-aggregation (83.13%), hydrophobicity (75.45–81.77%) antioxidant activity (48.86%), and antimicrobial activity (15–17 mm). Based on 16S <i>rRNA</i> gene analysis, CF-7 was identified as <i>Lactiplantibacillus plantarum</i>. Whole genome analysis of <i>L. plantarum</i> CF-7 revealed a single circular chromosome of 3.15 Mb with a GC content of 44.45%. The genome contained 2903 protein-coding genes with functional annotation indicating a predominance of genes related to metabolism and transcription. Average nucleotide identity (ANI), and core gene analysis showed similarity to other <i>L. plantarum</i> strains. Comparative genome analysis identified 85 unique genes. Gene cluster analyses revealed the presence of bacteriocin biosynthetic and other antimicrobial associated genes. The drug resistance and virulence factor database showed the presence of only vancomycin resistance gene and no true virulence factors. These findings indicate that <i>L. plantarum</i> CF-7 exhibits strong probiotic potential and can be considered safe for potential application in the food industry.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phenotypic and Whole Genome Analysis Depicts the Probiotic Potential of Lactiplantibacillus plantarum CF-7 Isolated from the Calf’s Gut

  • 期刊论文

  • 发表日期：

    2025年11月26日

查看全文
  • Asiya Batool,
  • Humaira Yasmin,
  • Muhammad Nadeem Hassan

摘要

Abstract

Lactic acid bacteria (LAB) are ubiquitous probiotics which provide health benefits to the host when consumed in optimal concentrations. Lactobacillus spp., an essential member of the Lactic acid bacteria (LAB), are widely recognized as safe. An indigenous probiotic of the same ecological niche can be more effective due to its environmental compatibility and high adaptability. This study aims to assess the probiotic potential and genetic diversity of an indigenous strain CF-7, isolated from a domestically reared Sahiwal calf by phenotypic and whole genome analysis. Strain CF-7 exhibited desirable probiotic traits, including gastrointestinal tolerance (85.6–95.1%) auto-aggregation (83.13%), hydrophobicity (75.45–81.77%) antioxidant activity (48.86%), and antimicrobial activity (15–17 mm). Based on 16S rRNA gene analysis, CF-7 was identified as Lactiplantibacillus plantarum. Whole genome analysis of L. plantarum CF-7 revealed a single circular chromosome of 3.15 Mb with a GC content of 44.45%. The genome contained 2903 protein-coding genes with functional annotation indicating a predominance of genes related to metabolism and transcription. Average nucleotide identity (ANI), and core gene analysis showed similarity to other L. plantarum strains. Comparative genome analysis identified 85 unique genes. Gene cluster analyses revealed the presence of bacteriocin biosynthetic and other antimicrobial associated genes. The drug resistance and virulence factor database showed the presence of only vancomycin resistance gene and no true virulence factors. These findings indicate that L. plantarum CF-7 exhibits strong probiotic potential and can be considered safe for potential application in the food industry.