Scholarlink

Abstract <p>This study evaluated the protective effects of rutin against chromium (VI) [Cr(VI)]-induced hematotoxicity, histopathological changes, genotoxicity and gene expression alterations in <i>Ctenopharyngodon idellus</i>. Chromium exposure (C3 group) resulted in a significant, time-dependent increase in chromium accumulation in blood cells, reaching a 10.56-fold increase in erythrocytic abnormalities by T3. Rutin supplementation (C4 group) significantly reduced chromium accumulation, with reductions of 0.21-fold at T1 and T2, and decreased erythrocytic abnormalities by 46.04% at T3. Histopathological analysis revealed prominent erythrocytic anomalies such as echinocytes, spherocytes, and dacrocytes in the C3 group, markedly mitigated in the C4 group. The comet assay revealed double-stranded DNA damage in grade 3, the percentage of which declined by 1.5-fold with rutin. Transcriptional profiling demonstrated significant upregulation of <i>p53</i> and <i>Nrf2</i> and downregulation of <i>Mt2</i> in the C3 group, indicative of oxidative stress, DNA damage, and impaired detoxification. Rutin supplementation normalized <i>p53</i> expression by 2–36% and <i>Nrf2</i> expression by 16.7–29.4% at T2 and T3 while partially restoring <i>Mt2</i> expression by 18.2 and 20.5% at T1 and T3. The results suggest that rutin’s antioxidant properties, including its ability to chelate heavy metals and enhance metallothionein-mediated detoxification, contribute to reduced oxidative damage and improved cellular defense. These findings highlight rutin as a promising dietary supplement for mitigating heavy metal toxicity in aquatic organisms, with implications for environmental and aquaculture management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Protective Role of Rutin against Chromium(VI) Toxicity in Fish: Insights from Hematotoxicity, Histopathology, and Gene Expression

  • 期刊论文

  • 发表日期：

    2025年9月5日

查看全文
  • Kriti Handa,
  • Aashaq Hussain Bhat,
  • Dalia Fouad,
  • Rajinder Jindal

摘要

Abstract

This study evaluated the protective effects of rutin against chromium (VI) [Cr(VI)]-induced hematotoxicity, histopathological changes, genotoxicity and gene expression alterations in Ctenopharyngodon idellus. Chromium exposure (C3 group) resulted in a significant, time-dependent increase in chromium accumulation in blood cells, reaching a 10.56-fold increase in erythrocytic abnormalities by T3. Rutin supplementation (C4 group) significantly reduced chromium accumulation, with reductions of 0.21-fold at T1 and T2, and decreased erythrocytic abnormalities by 46.04% at T3. Histopathological analysis revealed prominent erythrocytic anomalies such as echinocytes, spherocytes, and dacrocytes in the C3 group, markedly mitigated in the C4 group. The comet assay revealed double-stranded DNA damage in grade 3, the percentage of which declined by 1.5-fold with rutin. Transcriptional profiling demonstrated significant upregulation of p53 and Nrf2 and downregulation of Mt2 in the C3 group, indicative of oxidative stress, DNA damage, and impaired detoxification. Rutin supplementation normalized p53 expression by 2–36% and Nrf2 expression by 16.7–29.4% at T2 and T3 while partially restoring Mt2 expression by 18.2 and 20.5% at T1 and T3. The results suggest that rutin’s antioxidant properties, including its ability to chelate heavy metals and enhance metallothionein-mediated detoxification, contribute to reduced oxidative damage and improved cellular defense. These findings highlight rutin as a promising dietary supplement for mitigating heavy metal toxicity in aquatic organisms, with implications for environmental and aquaculture management.