Abstract <p>In this study, leaf extracts from various plants such as Lemon (<i>Citrus limon</i>), Banana (<i>Musa balbisiana</i>), Neem (<i>Azadirachta indica</i>), <i>Aloe vera</i> (<i>Aloe barbadensis</i>), Hibiscus (<i>Hibiscus rosa-sinensis</i>), Mint (<i>Mentha arvensis</i>) and Coriander (<i>Coriandrum sativum</i>) were prepared for the development of silver nanoparticles (AgNPs). Their formation was confirmed by UV-Vis spectrophotometer (400–430 nm) whereas their morphology was examined by Atomic Force Microscopy (AFM) presenting a variety of different shapes ranging from irregular to elliptical. Zeta sizer assessed their size (78.54–633.51 nm) and distribution (100%) produced after using plant leaf extracts. The smallest size was produced by Coriander (78.54 ± 0.12 nm) with spherical morphology enclosing a zeta potential of –9.91 mV while, X-Ray Diffraction (XRD) validated its crystalline nature. The antibacterial properties were examined against <i>Klebsiella pneumoniae</i> (BTCB04), <i>Acinetobacter</i> sp. (BTCB05) and <i>Staphylococcus aureus</i> (BTCB02) with concentrations of AgNPs between 3–7 µg/mL signifying efficient outcome with plant-leaf extracts of coriander which established the smallest AgNPs. A maximum zone of inhibition (ZOI) with <i>Klebsiella pneumoniae</i> (BTCB04) at 32.5 ± 0.18 mm was detected which can be of use for medicinal studies in future.</p>

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Green Synthesis of Silver Nanoparticles Using Medicinal Plant-Leaf Extracts: An Antibacterial Action

  • A. Shahzad

摘要

Abstract

In this study, leaf extracts from various plants such as Lemon (Citrus limon), Banana (Musa balbisiana), Neem (Azadirachta indica), Aloe vera (Aloe barbadensis), Hibiscus (Hibiscus rosa-sinensis), Mint (Mentha arvensis) and Coriander (Coriandrum sativum) were prepared for the development of silver nanoparticles (AgNPs). Their formation was confirmed by UV-Vis spectrophotometer (400–430 nm) whereas their morphology was examined by Atomic Force Microscopy (AFM) presenting a variety of different shapes ranging from irregular to elliptical. Zeta sizer assessed their size (78.54–633.51 nm) and distribution (100%) produced after using plant leaf extracts. The smallest size was produced by Coriander (78.54 ± 0.12 nm) with spherical morphology enclosing a zeta potential of –9.91 mV while, X-Ray Diffraction (XRD) validated its crystalline nature. The antibacterial properties were examined against Klebsiella pneumoniae (BTCB04), Acinetobacter sp. (BTCB05) and Staphylococcus aureus (BTCB02) with concentrations of AgNPs between 3–7 µg/mL signifying efficient outcome with plant-leaf extracts of coriander which established the smallest AgNPs. A maximum zone of inhibition (ZOI) with Klebsiella pneumoniae (BTCB04) at 32.5 ± 0.18 mm was detected which can be of use for medicinal studies in future.