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Abstract <p>Pebrine caused by <i>Nosema bombycis</i> infection is one of the most threatening diseases to sericulture. Trehalose-6-phosphate synthetase is a key rate-limiting enzyme in the trehalose metabolism pathway. <i>N.&#xa0;bombycis</i> trehalose phosphate synthetase 2 (NbTPS2) is a subcellular unit of <i>N. bombycis</i> trehalose synthase gene complex. Here, <i>NbTPS2</i> gene was amplified, sequenced and expressed in <i>E. coli</i> BL21 strain. The result showed that <i>NbTPS2</i> gene contains an open reading frame with length of 1371 bp and encoded a protein with molecular mass of approximately 57.87 kDa. His pull-down result showed that 66 <i>N. bombycis</i> proteins and 359 silkworm proteins may be directly or indirectly interacted with NbTPS2. Molecular phylogeny analysis result showed that <i>NbTPS2</i> gene was conservative in different microsporidia. In addition, the highest relative transcription level of <i>NbTPS2</i> at 6 h after infection suggesting that NbTPS2 plays an important role in the early infection stage (possibly germination stage) for <i>N. bombycis</i>. These results revealed the molecular characteristic of NbTPS2, laying the molecular foundation for the subsequent functional study of NbTPS2.</p>

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Molecular Characterization of Trehalose-6-Phosphate Synthetase 2 in Nosema bombycis

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • Min Ling,
  • Chenxiao Dong,
  • Haodong Yu,
  • Pai Liu,
  • Jiangfeng Shi,
  • Qiang Wang,
  • Xudong Tang,
  • Zhongyuan Shen,
  • Yiling Zhang

摘要

Abstract

Pebrine caused by Nosema bombycis infection is one of the most threatening diseases to sericulture. Trehalose-6-phosphate synthetase is a key rate-limiting enzyme in the trehalose metabolism pathway. N. bombycis trehalose phosphate synthetase 2 (NbTPS2) is a subcellular unit of N. bombycis trehalose synthase gene complex. Here, NbTPS2 gene was amplified, sequenced and expressed in E. coli BL21 strain. The result showed that NbTPS2 gene contains an open reading frame with length of 1371 bp and encoded a protein with molecular mass of approximately 57.87 kDa. His pull-down result showed that 66 N. bombycis proteins and 359 silkworm proteins may be directly or indirectly interacted with NbTPS2. Molecular phylogeny analysis result showed that NbTPS2 gene was conservative in different microsporidia. In addition, the highest relative transcription level of NbTPS2 at 6 h after infection suggesting that NbTPS2 plays an important role in the early infection stage (possibly germination stage) for N. bombycis. These results revealed the molecular characteristic of NbTPS2, laying the molecular foundation for the subsequent functional study of NbTPS2.