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Abstract <p><i>Dactylorhiza fuchsii</i> (Druce) Soó is a rare species listed in the Red Books of the Moscow Region and 25 regions of the Russian Federation. This study focuses on optimizing clonal micropropagation and conservation methods for <i>D. fuchsii</i> and identifying anatomical features of underground organs under in&#xa0;vitro and <i>ex vitro</i> conditions. The results showed that cultivating protocorms on 1/2 MS medium with 1.5 mg/L 6‑BAP and 1.5 mg/L IBA under light conditions is optimal for forming new protocorms. Conversely, 1/2 MS medium with 1.5 mg/L 6-BAP and 2.0 mg/L IBA under dark conditions promoted protocorm development and seedling formation. For seedling development, 1/2 MS medium with 1.0 mg/L 6-BAP and 100 mL/L coconut water was most effective. The highest numbers of protocorm-like bodies were obtained with 1/2 MS medium containing 2.0 mg/L KIN and 20 g/L potato homogenate. Seedlings were successfully adapted after three months of cold treatment at 5°C in the dark, followed by growth in a substrate of bark, perlite, sand, and peat moss. Anatomical analysis showed that increased root diameter results from an increase in the number of parenchyma layers and cell size, as well as an increase in the number and development of vascular bundles in the stele. Tuber anatomy was also studied for the first time under in vitro and <i>ex vitro</i> conditions. This study provides valuable insights for the large-scale propagation of this endangered species and their reintroduction into the environment to preserve biodiversity.</p>

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Micropropagation and Anatomical Features of Dactylorhiza fuchsii (Druce) Soó under Different Cultivation Conditions

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • M. Hussien,
  • O. I. Molkanova,
  • T. Y. Konovalova,
  • V. A. Koval

摘要

Abstract

Dactylorhiza fuchsii (Druce) Soó is a rare species listed in the Red Books of the Moscow Region and 25 regions of the Russian Federation. This study focuses on optimizing clonal micropropagation and conservation methods for D. fuchsii and identifying anatomical features of underground organs under in vitro and ex vitro conditions. The results showed that cultivating protocorms on 1/2 MS medium with 1.5 mg/L 6‑BAP and 1.5 mg/L IBA under light conditions is optimal for forming new protocorms. Conversely, 1/2 MS medium with 1.5 mg/L 6-BAP and 2.0 mg/L IBA under dark conditions promoted protocorm development and seedling formation. For seedling development, 1/2 MS medium with 1.0 mg/L 6-BAP and 100 mL/L coconut water was most effective. The highest numbers of protocorm-like bodies were obtained with 1/2 MS medium containing 2.0 mg/L KIN and 20 g/L potato homogenate. Seedlings were successfully adapted after three months of cold treatment at 5°C in the dark, followed by growth in a substrate of bark, perlite, sand, and peat moss. Anatomical analysis showed that increased root diameter results from an increase in the number of parenchyma layers and cell size, as well as an increase in the number and development of vascular bundles in the stele. Tuber anatomy was also studied for the first time under in vitro and ex vitro conditions. This study provides valuable insights for the large-scale propagation of this endangered species and their reintroduction into the environment to preserve biodiversity.