Scholarlink

Abstract <p>A wide range of molecular disorders leading to physiological damage in plants under drought stress can be attributed to the production of reactive oxygen species. Leaf samples were collected simultaneously from both water-stressed and control plants at 4, 8, 12, and 24 h post-drought treatment. It seems that SLM046 genotype reduces the amount of Nicotinamide adenine dinucleotide phosphate by decreasing the expression of <i>Ferredoxin-NADP reductase</i> and <i>Glucose-6-phosphate</i> to <i>ribulose-5-phosphate</i> genes in the early hours and increasing the expression of <i>NADPH-dependent thioredoxin reductase</i>, <i>Glutathione reductase</i> and <i>NADPH dehydrogenase</i> complex in the final hour. SLM046 genotype, in contrast to Hyola308, can decrease the ratio of NAD(P)H to NAD(P)+ and increase the Reduced glutathione to Oxidized glutathione ratio by increasing the expression of cytoplasmic <i>Glutathione reductase</i> gene, and hence prevent drought stress. Unlike Hyola308 genotype, <i>Peroxiredoxin,</i> <i>Thioredoxin</i>, and <i>Glutaredoxin</i> regulatory system are not primarily responsible for adjusting the cytoplasmic peroxides in SLM046. Results revealed that by increasing the expression of <i>Peroxiredoxin</i> gene, <i>Glutaredoxin</i> and <i>Thioredoxin</i> genes were up-regulated to convert the inactive oxidized <i>Peroxiredoxin</i> to its reduced activated form. Superoxide dismutase enzyme activity in drought-stressed SLM046 was higher than that of un-stressed genotype and more than those of stressed and un-stressed Hyola308 plants at 8 and 24 hours after applying the stress. During the initial hours, the enzyme activity increased with catalase activity in un-stressed SLM046 and Hyola308 genotypes. It decreased over time, whereas it exhibited an upwards trend under stress conditions, but the observed increase was more in Hyola308 genotype than SLM046.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Catalase and Superoxide Dismutase Kinetics and Expression Changes of Some Oxidoreductase Genes in Rapeseed Genotypes under Drought Stress

  • 期刊论文

  • 发表日期：

    2025年2月1日

查看全文
  • Mohammad Mohsenzadeh Golfazani,
  • Habibollah Samizadeh Lahiji,
  • Hassan Hassani Kumleh

摘要

Abstract

A wide range of molecular disorders leading to physiological damage in plants under drought stress can be attributed to the production of reactive oxygen species. Leaf samples were collected simultaneously from both water-stressed and control plants at 4, 8, 12, and 24 h post-drought treatment. It seems that SLM046 genotype reduces the amount of Nicotinamide adenine dinucleotide phosphate by decreasing the expression of Ferredoxin-NADP reductase and Glucose-6-phosphate to ribulose-5-phosphate genes in the early hours and increasing the expression of NADPH-dependent thioredoxin reductase, Glutathione reductase and NADPH dehydrogenase complex in the final hour. SLM046 genotype, in contrast to Hyola308, can decrease the ratio of NAD(P)H to NAD(P)+ and increase the Reduced glutathione to Oxidized glutathione ratio by increasing the expression of cytoplasmic Glutathione reductase gene, and hence prevent drought stress. Unlike Hyola308 genotype, Peroxiredoxin, Thioredoxin, and Glutaredoxin regulatory system are not primarily responsible for adjusting the cytoplasmic peroxides in SLM046. Results revealed that by increasing the expression of Peroxiredoxin gene, Glutaredoxin and Thioredoxin genes were up-regulated to convert the inactive oxidized Peroxiredoxin to its reduced activated form. Superoxide dismutase enzyme activity in drought-stressed SLM046 was higher than that of un-stressed genotype and more than those of stressed and un-stressed Hyola308 plants at 8 and 24 hours after applying the stress. During the initial hours, the enzyme activity increased with catalase activity in un-stressed SLM046 and Hyola308 genotypes. It decreased over time, whereas it exhibited an upwards trend under stress conditions, but the observed increase was more in Hyola308 genotype than SLM046.