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Abstract <p>This study aimed to assess the potential for crossbreeding between Susan-e-Chelcharagh (<i>Lilium ledebourii</i>), a rare native lilium species in Iran, and selected commercial lily cultivars. Initially, we compared the cytogenetic characteristics of <i>L. ledebourii</i> with several commercial cultivars, followed by crossbreeding attempts using both conventional and cut-style pollination methods. Additionally, we explored the possibility of embryo rescue through ovary slice culture in the maternal parent of the Brindisi cultivar when crossed with <i>L. ledebourii</i> as the paternal parent. Our results revealed that <i>L. ledebourii</i> possesses a diploid ploidy level (2<i>n</i>&#xa0;= 2<i>x</i> = 24) and exhibits high pollen viability (88.5%) and germination rates (77.3%). Crossbreeding endeavors yielded variable fruit formation and growth, with varying success rates in obtaining viable seeds. Notably, the cut-style pollination method proved ineffective in certain commercial cultivars, indicating the presence of pre- and post-fertilization barriers. Findings from the ovary slice culture of the Brindisi maternal parent crossed with <i>L. ledebourii</i> pollen indicated that sucrose and NAA concentrations significantly influenced the survival and enlargement of ovary slices. Optimal ovule germination was observed with 3% sucrose combined with 0.1 mg/L NAA, followed by treatment with 3% sucrose and 0.2 mg/L NAA. Histological analysis further elucidated the presence of post-fertilization barriers in specific crosses, leading to ovule degeneration and failed seed formation. These findings underscore the importance of considering genetic divergence, ploidy levels, and other pertinent factors when undertaking interspecies crosses and hybridization within the <i>Lilium</i> genus.</p>

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Exploring the Hybridization of Lilium ledebourii Boiss with Commercial Lily Cultivars and Using Embryo Rescue Potential

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • Younes Mahdavi Fikejvar,
  • Pejman Azadi

摘要

Abstract

This study aimed to assess the potential for crossbreeding between Susan-e-Chelcharagh (Lilium ledebourii), a rare native lilium species in Iran, and selected commercial lily cultivars. Initially, we compared the cytogenetic characteristics of L. ledebourii with several commercial cultivars, followed by crossbreeding attempts using both conventional and cut-style pollination methods. Additionally, we explored the possibility of embryo rescue through ovary slice culture in the maternal parent of the Brindisi cultivar when crossed with L. ledebourii as the paternal parent. Our results revealed that L. ledebourii possesses a diploid ploidy level (2n = 2x = 24) and exhibits high pollen viability (88.5%) and germination rates (77.3%). Crossbreeding endeavors yielded variable fruit formation and growth, with varying success rates in obtaining viable seeds. Notably, the cut-style pollination method proved ineffective in certain commercial cultivars, indicating the presence of pre- and post-fertilization barriers. Findings from the ovary slice culture of the Brindisi maternal parent crossed with L. ledebourii pollen indicated that sucrose and NAA concentrations significantly influenced the survival and enlargement of ovary slices. Optimal ovule germination was observed with 3% sucrose combined with 0.1 mg/L NAA, followed by treatment with 3% sucrose and 0.2 mg/L NAA. Histological analysis further elucidated the presence of post-fertilization barriers in specific crosses, leading to ovule degeneration and failed seed formation. These findings underscore the importance of considering genetic divergence, ploidy levels, and other pertinent factors when undertaking interspecies crosses and hybridization within the Lilium genus.