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Abstract <p>This study aims to isolate <i>Aeromonas hydrophila</i> from the aquatic environment of Qarun Lake. Wild waterfowl, Nile tilapia, and water were collected from three different sites of Qarun Lake. Bacteriological identification revealed the isolation of <i>A. hydrophila</i> biochemically using API 20 E and NE. Molecular identification was conducted using the Aeromonas <i>16SrRNA</i> and <i>GyrB</i> genes that produce amplicons at 461 and 1100 bp., respectively, in all examined samples. Sequencing analysis of the retrieved <i>A. hydrophila</i> isolates from fish and wild waterfowl with accession numbers OR792158 and OR792159 respectively showed high identity and similarity with other <i>A. hydrophila</i> sequences listed in the GenBank database. The prevalence of infection with <i>A. hydrophila</i> was 50, 37.5 and 16.7% in wild waterfowl, tilapia fish and the lake water respectively. The physicochemical water analysis revealed an alteration in the levels of phosphate, ammonia, and nitrates. According to the results of the antibiogram, nearly all isolates of <i>A. hydrophila</i> were susceptible to fluoroquinolones and highly resistant to β-lactams and tetracyclines. In vivo infectivity test was conducted using <i>A.&#xa0;hydrophila</i> stains isolated from waterfowl revealing bacterial re-isolation from challenged fish. The histopathological analyses of experimentally infected fish tissues revealed pathological changes in gills, kidney, and liver. All the above-mentioned results support our hypothesis that <i>A. hydrophila</i> can be cross transmitted from wild migratory waterfowl to inhabitant fish of Lake Qarun under the influence of poor water quality parameters.</p>

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Assessment of the Role of Wild Waterfowl as Potential Vectors of Aeromonas hydrophila and Its Cross-Transmission to Qarun Lake’s Aquatic Environment, Considering the Altered Water Quality Parameters

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • D. A. Abdel-moneam,
  • H. S. Khalefa,
  • M. Shaalan,
  • E. A. Elshafiee,
  • Z. S. Ahmed

摘要

Abstract

This study aims to isolate Aeromonas hydrophila from the aquatic environment of Qarun Lake. Wild waterfowl, Nile tilapia, and water were collected from three different sites of Qarun Lake. Bacteriological identification revealed the isolation of A. hydrophila biochemically using API 20 E and NE. Molecular identification was conducted using the Aeromonas 16SrRNA and GyrB genes that produce amplicons at 461 and 1100 bp., respectively, in all examined samples. Sequencing analysis of the retrieved A. hydrophila isolates from fish and wild waterfowl with accession numbers OR792158 and OR792159 respectively showed high identity and similarity with other A. hydrophila sequences listed in the GenBank database. The prevalence of infection with A. hydrophila was 50, 37.5 and 16.7% in wild waterfowl, tilapia fish and the lake water respectively. The physicochemical water analysis revealed an alteration in the levels of phosphate, ammonia, and nitrates. According to the results of the antibiogram, nearly all isolates of A. hydrophila were susceptible to fluoroquinolones and highly resistant to β-lactams and tetracyclines. In vivo infectivity test was conducted using A. hydrophila stains isolated from waterfowl revealing bacterial re-isolation from challenged fish. The histopathological analyses of experimentally infected fish tissues revealed pathological changes in gills, kidney, and liver. All the above-mentioned results support our hypothesis that A. hydrophila can be cross transmitted from wild migratory waterfowl to inhabitant fish of Lake Qarun under the influence of poor water quality parameters.