Morphogenesis In Vitro in Calluses of Lavender Lavandula angustifolia Mill.: Histological Aspects

Histological events occurring in the calluses of Lavandula angustifolia Mill. at the initial stages of in vitro culture (one passage) were described for the first time. It was found that the non-morphogenic callus is mainly represented by parenchymal tissue with few morphogenetic foci, which are mostly degenerated. In morphogenic calluses the in vitro morphogenesis pathways such as de novo organogenesis and indirect in vitro somatic embryogenesis have been identified and multiple developing morphogenetic foci have also been noted. The question of the implementation of the pluri- and totipotency properties of callus cells in vitro is discussed. The histological data can be used in choosing the direction of application of regenerants obtained from calluses of this valuable essential oil and medicinal plant in various cell technologies.