Scholarlink

Abstract <p>This study presents a rapid and a highly sensitive method for determining seven major biologically active lignans—lyoniside, ssioside, hydroxymatairesinol, secoisolariciresinol, nortrachelogenin, pinoresinol, and matairesinol—in plant extracts by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in the multiple reaction monitoring mode. Analytes ionization under electrospray ionization and atmospheric pressure chemical ionization conditions was studied. We analyzed tandem mass spectra of selected precursor ions and identified the main pathways of their collision-induced dissociation (CID). The optimization of reversed-phase HPLC separation parameters and mass spectrometric detection enabled the development of a method for the simultaneous determination of the analytes in plant extracts, achieving an analysis time of less than 6 min and detection limits ranging from 0.72 to 9.0 μg/L. The developed approach was validated and tested using extracts from compression wood of coniferous and deciduous trees, stems of wild rosemary, and above-ground parts of several berry plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Rapid Determination of Lignans in Plant Raw Materials by Liquid Chromatography–Tandem Mass Spectrometry

  • 期刊论文

  • 发表日期：

    2025年12月22日

查看全文
  • D. I. Falev,
  • I. S. Voronov,
  • A. A. Onuchina,
  • A. V. Faleva,
  • D. S. Kosyakov,
  • N. V. Ulyanovskii

摘要

Abstract

This study presents a rapid and a highly sensitive method for determining seven major biologically active lignans—lyoniside, ssioside, hydroxymatairesinol, secoisolariciresinol, nortrachelogenin, pinoresinol, and matairesinol—in plant extracts by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in the multiple reaction monitoring mode. Analytes ionization under electrospray ionization and atmospheric pressure chemical ionization conditions was studied. We analyzed tandem mass spectra of selected precursor ions and identified the main pathways of their collision-induced dissociation (CID). The optimization of reversed-phase HPLC separation parameters and mass spectrometric detection enabled the development of a method for the simultaneous determination of the analytes in plant extracts, achieving an analysis time of less than 6 min and detection limits ranging from 0.72 to 9.0 μg/L. The developed approach was validated and tested using extracts from compression wood of coniferous and deciduous trees, stems of wild rosemary, and above-ground parts of several berry plants.