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Abstract <p>This paper describes a two-step algorithm for detecting muscarine, ibotenic acid, and muscimol in urine samples. A two-step procedure was developed for detecting muscarine, muscimol, and ibotenic acid using a data-dependent mode of MRM analysis with the simultaneous full mass spectrum recording of the target substances. Analytes were detected by HPLC coupled with a triple quadrupole mass spectrometric detector (LC–MS/MS Shimadzu 8050). Chromatographic separation was performed in a Phenomenex Kinetex C18 reversed-phase column. At the first step, muscarine was detected in a diluted urine sample. At the second step, muscimol and ibotenic acid were found using a bimolecular dansylation method. The procedure has been successfully applied to routine chemical and toxicological analyses of urine samples from patients suspected of mushroom poisoning.</p>

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HPLC–MS/MS Methods for Detecting Muscarine, Muscimol, and Ibotenic Acid in Fungal Samples and Biological Matrices

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • M. Sh. Aigumov,
  • A. P. Novikov,
  • M. V. Vishnevskii,
  • N. A. Chernova,
  • I. V. Maidanets,
  • M. A. Gofenberg,
  • D. V. Kuznetsov,
  • N. V. Samyshkina,
  • L. N. Rizvanova,
  • A. Z. Temerdashev,
  • S. A. Savchuk

摘要

Abstract

This paper describes a two-step algorithm for detecting muscarine, ibotenic acid, and muscimol in urine samples. A two-step procedure was developed for detecting muscarine, muscimol, and ibotenic acid using a data-dependent mode of MRM analysis with the simultaneous full mass spectrum recording of the target substances. Analytes were detected by HPLC coupled with a triple quadrupole mass spectrometric detector (LC–MS/MS Shimadzu 8050). Chromatographic separation was performed in a Phenomenex Kinetex C18 reversed-phase column. At the first step, muscarine was detected in a diluted urine sample. At the second step, muscimol and ibotenic acid were found using a bimolecular dansylation method. The procedure has been successfully applied to routine chemical and toxicological analyses of urine samples from patients suspected of mushroom poisoning.