Development and Validation of a Simple, Precise, and Robust Reversed-Phase High-Performance Liquid Chromatography-Ultraviolet Method for the Determination of Polyvinylpyrrolidone in Adhesives

Polyvinylpyrrolidone (PVP) is available in a range of molecular weights and related viscosities that can be selected according to the desired application properties. A simple, accurate, and robust reversed-phase liquid chromatography method has been developed and subsequently validated for the estimation of polyvinylpyrrolidone K-90 in adhesive formulations. Separation and maximum response of the peak were obtained at UV a wavelength of 200 nm on a Zorbax Eclipse XDB C18 column. The limit of detection and limit of quantification of PVP were found to be 16.0 and 50.0 mg/L, respectively. The calibration curve showed good linearity over the concentration range of 50.0 to 1000.0 mg/L, with a correlation coefficient of 0.999. The proposed method was validated for specificity, linearity, accuracy, precision, robustness, and ruggedness, and successfully applied for the determination of PVP in glue samples.