Determination of Tioconazole via Spectrophotometry Using Charge Transfer Complex Formation

A new simple, precise, and sensitive charge transfer method for estimation of tioconazole drug in pure form and Gyno-Trosyd tablet was developed based on the reaction of the n-electron donor tioconazole drug with π-receptors, namely chloroanilic acid, 2,3-dichloro-5,6-dicyano-1,4-benzoquinon, and picric acid. The complexes were determined spectrophotometrically at 460, 402, and 520 nm for the tioconazole-2,3-dichloro-5,6-dicyano-1,4-benzoquinon, tioconazole-picric acid, and tioconazole-chloroanilic acid complexes, respectively. The conditions under which experiments should be conducted have been extensively studied. Beer’s law was obeyed over the working concentration ranges of 10–100, 10–250, and 2–140 μg/mL for tioconazole-2,3-dichloro-5,6-dicyano-1,4-benzoquinon, tioconazole-chloroanilic acid, and tioconazole-picric acid complexes, respectively.