Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy

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发表日期：

2024年6月1日

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M. A. Kryuchkova,
I. R. Ishmukhametov,
Y. A. Frank,
A. V. Simakova,
V. V. Yartsev,
D. A. Nadueva,
A. A. Varenitsina,
Y. V. Andreeva,
R. F. Fakhrullin

摘要

Abstract

Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.