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Abstract <p>The blue shark (<i>Prionace glauca</i>) is one of the main targets in the global shark fin trade. In recent years, the resources of <i>P. glauca</i> have declined dramatically, and it has been listed as a “Near Threatened” species by the IUCN Red List. Till now, limited studies related to the genomics of <i>P. glauca</i> have been reported, which should constrain conservation genetics studies of this species. In this study, we collected the sample of <i>P. glauca</i> off Zhoushan, China, and carried out genome-wide investigations to evaluate the fundamental genomic characteristics and analyze the genome-wide distribution pattern of microsatellites. The results showed that the genome size of <i>P. glauca</i> was about 3 Gb, the heterozygosity rate was 0.54%, and the proportion of repeat sequences was 65.06%. The preliminary assembly results of the genome showed that the Contig N50 was 2357 bp and the Scaffold N50 was 3917 bp. A total of 444 196 microsatellite loci were screened from the preliminary assembly of <i>P. glauca</i>, with a relative abundance of 172.23 loci/Mb. Among them, the number of dinucleotide microsatellites was the largest, accounting for 50.34% of the total microsatellites. The C/G and AC/GT types were dominant in mononucleotide and dinucleotide repeats, accounting for 27.57 and 26.22%, respectively. The results of this study can provide basic data for high-quality genome sequencing of <i>P. glauca</i>, and the detected microsatellite loci can provide molecular markers for further conservation genetics and evolutionary biology studies of <i>P. glauca</i>.</p>

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Analysis of Genomic Characteristics and Genome-Wide Microsatellite Distribution Pattern of the Blue Shark (Prionace glauca)

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • M. S. Li,
  • Z. Y. Qiu,
  • T. Wang,
  • Y. Y. Sun,
  • C. C. Hu,
  • S. Y. Xu

摘要

Abstract

The blue shark (Prionace glauca) is one of the main targets in the global shark fin trade. In recent years, the resources of P. glauca have declined dramatically, and it has been listed as a “Near Threatened” species by the IUCN Red List. Till now, limited studies related to the genomics of P. glauca have been reported, which should constrain conservation genetics studies of this species. In this study, we collected the sample of P. glauca off Zhoushan, China, and carried out genome-wide investigations to evaluate the fundamental genomic characteristics and analyze the genome-wide distribution pattern of microsatellites. The results showed that the genome size of P. glauca was about 3 Gb, the heterozygosity rate was 0.54%, and the proportion of repeat sequences was 65.06%. The preliminary assembly results of the genome showed that the Contig N50 was 2357 bp and the Scaffold N50 was 3917 bp. A total of 444 196 microsatellite loci were screened from the preliminary assembly of P. glauca, with a relative abundance of 172.23 loci/Mb. Among them, the number of dinucleotide microsatellites was the largest, accounting for 50.34% of the total microsatellites. The C/G and AC/GT types were dominant in mononucleotide and dinucleotide repeats, accounting for 27.57 and 26.22%, respectively. The results of this study can provide basic data for high-quality genome sequencing of P. glauca, and the detected microsatellite loci can provide molecular markers for further conservation genetics and evolutionary biology studies of P. glauca.