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Abstract <p>The use of medicinal plants has been widespread since the beginning of human civilization. This research aimed at evaluating the level of genetic diversity and population structure of different species, subspecies and certain cultivated varieties of Iranian native <i>Origanum</i> L. (Lamiaceae). Twelve sequence related amplified polymorphism (SRAP) primer pairs and nine start codon targeted (SCoT) primers were used for studying a collection of 12 sampled populations belonging to three species of <i>O. vulgare</i>, <i>O. majorana</i> and <i>O.&#xa0;laevigatum</i>. The SRAP and SCoT primers amplified a total of 135 and 109 bands, respectively out of which 133 and 108 were polymorphic. The Me5-Em4 and SCoT-18 were found to be orderly the most efficient SRAP and SCoT primers to investigate the genetic diversity of <i>Origanum</i> germplasm. The results of cluster analysis by the data obtained by both maker systems alone or in combination, which were also supported by Principal Coordinate Analysis (PCoA), divided the studied populations into two main groups; one belonging to the central region and the second to the north-western regions of Iran. Analysis of Molecular Variance (AMOVA) showed that Iranian <i>Origanum</i> populations have high genetic diversity and gene flow in all geographical regions, where the contribution of intra-species and intra-regional variation is higher than the among-populations contribution. These results confirm the existence of a high variation in <i>Origanum</i> gene pool that could be exploited in breeding programs of the species and subspecies of this pharmaceutically important medicinal plant.</p>

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Genetic Diversity in Iranian Origanum L. (Lamiaceae) Populations in Relationship to Geographic Distribution as Revealed by SRAP and SCoT Molecular Markers

  • 期刊论文

  • 发表日期：

    2025年6月26日

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  • S. Tarkesh Esfahani,
  • S. Salesi,
  • A. Rezaei,
  • A. Mosleh Arani

摘要

Abstract

The use of medicinal plants has been widespread since the beginning of human civilization. This research aimed at evaluating the level of genetic diversity and population structure of different species, subspecies and certain cultivated varieties of Iranian native Origanum L. (Lamiaceae). Twelve sequence related amplified polymorphism (SRAP) primer pairs and nine start codon targeted (SCoT) primers were used for studying a collection of 12 sampled populations belonging to three species of O. vulgare, O. majorana and O. laevigatum. The SRAP and SCoT primers amplified a total of 135 and 109 bands, respectively out of which 133 and 108 were polymorphic. The Me5-Em4 and SCoT-18 were found to be orderly the most efficient SRAP and SCoT primers to investigate the genetic diversity of Origanum germplasm. The results of cluster analysis by the data obtained by both maker systems alone or in combination, which were also supported by Principal Coordinate Analysis (PCoA), divided the studied populations into two main groups; one belonging to the central region and the second to the north-western regions of Iran. Analysis of Molecular Variance (AMOVA) showed that Iranian Origanum populations have high genetic diversity and gene flow in all geographical regions, where the contribution of intra-species and intra-regional variation is higher than the among-populations contribution. These results confirm the existence of a high variation in Origanum gene pool that could be exploited in breeding programs of the species and subspecies of this pharmaceutically important medicinal plant.