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Abstract <p>Blackberries have garnered significant attention due to the nutritional richness, health benefits, and economic value of their fruits. In this study, a <i>NAC</i> gene was cloned and named <i>RuNAC43</i>, and its expression characteristics were analyzed. Sequence analysis revealed that its encoded protein contains a typical N-terminal conserved domain and a C-terminal transcriptional regulatory region. Bioinformatics analysis predicted that RuNAC43 is a hydrophilic and unstable protein localized to the plasma membrane, with its secondary structure predominantly composed of random coils. Phylogenetic analysis showed that RuNAC43 is most closely related to its homolog in wild strawberry, with a similarity of 85.5%. Expression pattern analysis indicated that the expression level of RuNAC43 significantly decreases during blackberry fruit development as maturation progresses. To investigate its function, an overexpression vector was constructed and introduced into Arabidopsis thaliana. The results demonstrated that overexpression of <i>RuNAC43</i> significantly increased cellulose content in the leaves of transgenic plants while reducing cellulase activity. In summary, this study preliminarily confirms that <i>RuNAC43</i> is involved in regulating fruit ripening and softening by positively regulating cellulose accumulation and negatively regulating cell wall degradation. This provides new genetic resources and a theoretical basis for elucidating the molecular mechanisms underlying blackberry fruit softening.</p>

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Cloning and Functional Analysis of Transcription Factor RuNAC43 Gene from Blackberry

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • H. Liu,
  • F. Zhao,
  • L. Lyu,
  • W. Wu,
  • J. Tian,
  • Y. Wu,
  • W. Li

摘要

Abstract

Blackberries have garnered significant attention due to the nutritional richness, health benefits, and economic value of their fruits. In this study, a NAC gene was cloned and named RuNAC43, and its expression characteristics were analyzed. Sequence analysis revealed that its encoded protein contains a typical N-terminal conserved domain and a C-terminal transcriptional regulatory region. Bioinformatics analysis predicted that RuNAC43 is a hydrophilic and unstable protein localized to the plasma membrane, with its secondary structure predominantly composed of random coils. Phylogenetic analysis showed that RuNAC43 is most closely related to its homolog in wild strawberry, with a similarity of 85.5%. Expression pattern analysis indicated that the expression level of RuNAC43 significantly decreases during blackberry fruit development as maturation progresses. To investigate its function, an overexpression vector was constructed and introduced into Arabidopsis thaliana. The results demonstrated that overexpression of RuNAC43 significantly increased cellulose content in the leaves of transgenic plants while reducing cellulase activity. In summary, this study preliminarily confirms that RuNAC43 is involved in regulating fruit ripening and softening by positively regulating cellulose accumulation and negatively regulating cell wall degradation. This provides new genetic resources and a theoretical basis for elucidating the molecular mechanisms underlying blackberry fruit softening.