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Abstract <p>Amino acids play active roles in plant development and perform various plant functions, including signaling molecules, controlling root and shoot architecture, flowering timing, and stress defense. The current study aimed to assess the effectiveness of exogenously applied amino acids as a pre-sowing seed treatment on drought tolerance of two canola (<i>Brassica napus</i> L.) cultivars. The seeds of the two canola cultivars, Punjab and Rachna, were pre-treated with varying levels of L-methionine (Met) and proline (Pro), i.e. control (no treatment), Met (25 mg/L), Pro (4605.2 mg/L), and Met + Pro (25 mg/L + 4605.2 mg/L). Fifteen-day-old seedlings raised from amino acid-pre-treated seeds were exposed to water stress. Drought significantly decreased plant growth (fresh and dry weights of the shoots and roots, lengths and leaf area/plant), chlorophyll contents, total soluble proteins, ascorbic acid, and yield-related traits (dry-fresh weight of siliquae, number of seeds/siliqua, number of siliquae/plant and total number of siliquae) of both canola cultivars. At the same time, it increased the relative membrane permeability (RMP), the activities of enzymes, and the contents of proline, glycine betaine (GB), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and malondialdehyde (MDA) in plants of both canola cultivars. Exogenous application of amino acids as pre-sowing seed treatment was effective in promoting growth and key metabolic processes and of varying levels of amino acids, the combination of Pro + Met considerably improved the leaf area, shoot and root fresh and dry weights, and lengths, chlorophyll content (<i>a</i>, <i>b</i>), proline, GB, total phenolics, ascorbic acid, total soluble proteins, enzymatic antioxidants, and yield-related characteristics, but in contrast it lowered down the accumulation of H<sub>2</sub>O<sub>2</sub> and MDA. The correlations between these characteristics were verified and overall, the findings demonstrated that although all amino acid pre-sowing seed treatments were effective in promoting growth and key biochemical processes in both canola cultivars.</p>

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Pre-Sowing Application of Methionine and Proline Mitigates Drought Stress in Two Canola (Brassica napus L.) Cultivars

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • S. Umer,
  • N. A. Akram,
  • M. Ashraf,
  • A. A. Abdel Latef

摘要

Abstract

Amino acids play active roles in plant development and perform various plant functions, including signaling molecules, controlling root and shoot architecture, flowering timing, and stress defense. The current study aimed to assess the effectiveness of exogenously applied amino acids as a pre-sowing seed treatment on drought tolerance of two canola (Brassica napus L.) cultivars. The seeds of the two canola cultivars, Punjab and Rachna, were pre-treated with varying levels of L-methionine (Met) and proline (Pro), i.e. control (no treatment), Met (25 mg/L), Pro (4605.2 mg/L), and Met + Pro (25 mg/L + 4605.2 mg/L). Fifteen-day-old seedlings raised from amino acid-pre-treated seeds were exposed to water stress. Drought significantly decreased plant growth (fresh and dry weights of the shoots and roots, lengths and leaf area/plant), chlorophyll contents, total soluble proteins, ascorbic acid, and yield-related traits (dry-fresh weight of siliquae, number of seeds/siliqua, number of siliquae/plant and total number of siliquae) of both canola cultivars. At the same time, it increased the relative membrane permeability (RMP), the activities of enzymes, and the contents of proline, glycine betaine (GB), hydrogen peroxide (H2O2), and malondialdehyde (MDA) in plants of both canola cultivars. Exogenous application of amino acids as pre-sowing seed treatment was effective in promoting growth and key metabolic processes and of varying levels of amino acids, the combination of Pro + Met considerably improved the leaf area, shoot and root fresh and dry weights, and lengths, chlorophyll content (a, b), proline, GB, total phenolics, ascorbic acid, total soluble proteins, enzymatic antioxidants, and yield-related characteristics, but in contrast it lowered down the accumulation of H2O2 and MDA. The correlations between these characteristics were verified and overall, the findings demonstrated that although all amino acid pre-sowing seed treatments were effective in promoting growth and key biochemical processes in both canola cultivars.