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Abstract <p>In the present study, potential allelopathic effects of soybean sprouts extract (SSE) on branched broomrape [<i>Philpanche ramosa</i> L. (<i>Pr</i>) syn. <i>Orobanche ramosa</i>] germination was investigated. We observed that using an optimised [50% (v/v)] concentration of SSE (referred as SSE<sub>50</sub>) as a pre-conditioning/priming-agent, an induced germination of <i>Pr</i> in concomitant with <i>de novo</i> RNA synthesis and a gene-specific enhanced expression of early germination marker-gene, abscisic acid 8'-hydroxylase (<i>PrCYP707A1</i>) was observed. Subsequent experiments were conducted to investigate the potential transcriptional and post-transcriptional alterations of <i>PrCYP707A1</i> linked with SSE<sub>50</sub> induced germination of <i>Pr</i>. Our findings from run-on and mRNA chase assays indicated that SSE<sub>50</sub>-primed <i>Pr</i> seeds showed a relatively enhanced transcriptional rate of <i>PrCYP707A1</i>, without affecting its mRNA turnover. Overall, SSE<sub>50</sub> is proposed as a inducer of <i>de novo</i> RNA synthesis as well as gene-specific transcriptional rate of <i>PrCYP707A1</i>, which could be further examined as a sustainable component in the management of broomrape infestations.</p>

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Transcriptional Activation, but Not mRNA Turnover of PrCYP707A1 is Linked with Soybean (Glycine max L.) Sprouts Extract Induced Germination of Obligate Root Parasitic Weed, Branched Broomrape (Philpanche ramosa L.)

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • R. Manoharlal,
  • M. T. Pavan,
  • H. N. Devika,
  • A. Bajpeyi,
  • L. Duhan,
  • R. Pasrija

摘要

Abstract

In the present study, potential allelopathic effects of soybean sprouts extract (SSE) on branched broomrape [Philpanche ramosa L. (Pr) syn. Orobanche ramosa] germination was investigated. We observed that using an optimised [50% (v/v)] concentration of SSE (referred as SSE50) as a pre-conditioning/priming-agent, an induced germination of Pr in concomitant with de novo RNA synthesis and a gene-specific enhanced expression of early germination marker-gene, abscisic acid 8'-hydroxylase (PrCYP707A1) was observed. Subsequent experiments were conducted to investigate the potential transcriptional and post-transcriptional alterations of PrCYP707A1 linked with SSE50 induced germination of Pr. Our findings from run-on and mRNA chase assays indicated that SSE50-primed Pr seeds showed a relatively enhanced transcriptional rate of PrCYP707A1, without affecting its mRNA turnover. Overall, SSE50 is proposed as a inducer of de novo RNA synthesis as well as gene-specific transcriptional rate of PrCYP707A1, which could be further examined as a sustainable component in the management of broomrape infestations.