Abstract <p>Fall armyworm (<i>Spodoptera frugiperda</i>) is a major pest affecting maize, the third most important cereal crop globally, causing significant yield losses. To mitigate this stress, nutrient translocation is a key strategy of plants, though it remains underexplored. Previous studies have highlighted the role of endophytic microbes in defence priming. In this context, the present investigation explores the nutrient mobilization strategies and root exudate pattern modifications in maize inoculated with <i>Bacillus amyloliquefaciens</i> (MZ895491) and <i>Glomus intraradices</i>. The results showed a significant increase in key nutrients, including chlorophyll, carbohydrates, and starch, in the systemic leaves of maize (<i>Zea mays</i> L.) (35 days old) infested by <i>S. frugiperda</i> irrespective of treatment. Additionally, nitrogen accumulation was notably higher in localized leaves, suggesting a targeted defense response against the pest. However, endophytes inoculation did not significantly influence nutrient mobilization. The analysis of root exudates revealed a higher secretion of fatty acids in endophytes inoculated treatments, particularly diisooctyl phthalate, which is known for its larvicidal properties. The concentration of fatty acids was higher in endophyte-inoculated plants compared to the uninoculated control. Furthermore, maize inoculated with endophytes produced unique defense metabolites in response to <i>S. frugiperda</i> attack. In conclusion, while endophytes had minimal impact on nutrient mobilization, they played a significant role in defense priming by altering root exudate patterns.</p>