Scholarlink

Abstract <p>A study into the effect of light conditions on the activity of DNA methyltransferases in maize leaves indicates that the active form of phytochrome A exhibits an inhibitory effect on the rate of functioning of CG DNA methyltransferases. Phytochrome regulation of the activity of site-dependent DNA methyltransferases leads to a change in the distribution of methylated cytosine in symmetric and asymmetric sites. Methyl-dependent restriction indicates an intensification of the work of CNG-DNA methyltransferases, which is manifested in an increase in the products of specific nuclease cleavage of genomic DNA in maize leaves when they are irradiated with red light. Phytochrome A plays a main role in regulating the activity and level of gene transcripts <i>MET1</i> and <i>MET4</i> CG DNA methyltransferases. However, phytochrome B acts as a regulator for methyltransferases that methylate CNG and CNN sites. An important argument in this conclusion is the reversible conversion of phytochrome B in response to sequential irradiation of plants with red and far-red light, which is not typical for phytochrome A.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The Role of DNA Methyltransferases of Different Families in Changing the Methyl Status of DNA in Corn Leaves under Irradiation with Light of Different Wavelengths

  • 期刊论文

  • 发表日期：

    2025年2月1日

查看全文
  • D. N. Fedorin,
  • A. T. Eprintsev

摘要

Abstract

A study into the effect of light conditions on the activity of DNA methyltransferases in maize leaves indicates that the active form of phytochrome A exhibits an inhibitory effect on the rate of functioning of CG DNA methyltransferases. Phytochrome regulation of the activity of site-dependent DNA methyltransferases leads to a change in the distribution of methylated cytosine in symmetric and asymmetric sites. Methyl-dependent restriction indicates an intensification of the work of CNG-DNA methyltransferases, which is manifested in an increase in the products of specific nuclease cleavage of genomic DNA in maize leaves when they are irradiated with red light. Phytochrome A plays a main role in regulating the activity and level of gene transcripts MET1 and MET4 CG DNA methyltransferases. However, phytochrome B acts as a regulator for methyltransferases that methylate CNG and CNN sites. An important argument in this conclusion is the reversible conversion of phytochrome B in response to sequential irradiation of plants with red and far-red light, which is not typical for phytochrome A.