Developing and Implementing a Fermentation System for Cultivating Methane-Oxidizing Bacteria

Based on theoretical research and laboratory tests, an original design of a jet-type bioreactor designed for growing methane-oxidizing bacteria was developed. A series of tests were conducted that demonstrate the unit efficiency, providing required cultivation productivity with the specified energy consumption for producing biomass. The main operating parameters of the fermentation unit (pH, temperature, pressure, the composition of the gas nutrients, and the flow rate inside the apparatus) were given, and problems associated with the limits on their permissible values (particularly the pressure effect and the composition of oxygen-containing gas on the cultivation behavior) were discussed. The effectiveness of using special structural elements in bioreactors was confirmed, which were designed to create a hydrodynamic regime that provide the necessary conditions for culture growth.