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Abstract <p>A one-step biotechnological method based on the microbial conversion of plant sterols using a transgenic strain originated from the <i>Mycolicibacterium neoaurum</i> strain, co-expressing genes encoding 17β-hydroxysteroid dehydrogenase from the fungus <i>Cochliobolus lunatus</i> and glucose-6-phosphate dehydrogenase from the bacterium <i>Mycobacterium tuberculosis</i> under the control of the acetamide-regulated promoter [P<sub><i>ami</i></sub>], allows for the phytosterols conversion with accumulation of the valuable 17β-reduced C<sub>19</sub>-steroids: testosterone and its ∆<sup>1</sup>-analogue. Optimization of bioconversion conditions for phytosterols at a high concentration (20 g/L) using a transgenic <i>M. neoaurum</i>-based strain, including the selection of solubilizing agents and optimization of inducer and glucose supplementation regimes, led to an increase in testosterone yield from 5.3&#xa0; to 6.9 g/L.</p>

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Optimization of the Phytosterol Bioconversion into the 17β-Reduced C19-Steroids by a Transgenic Mycolicibacterium neoaurum VKM Ac-1816D Strain

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • T. A. Timakova,
  • D. N. Tekucheva,
  • M. V. Karpov,
  • V. V. Fokina,
  • A. A. Shutov,
  • M. V. Donova

摘要

Abstract

A one-step biotechnological method based on the microbial conversion of plant sterols using a transgenic strain originated from the Mycolicibacterium neoaurum strain, co-expressing genes encoding 17β-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus and glucose-6-phosphate dehydrogenase from the bacterium Mycobacterium tuberculosis under the control of the acetamide-regulated promoter [Pami], allows for the phytosterols conversion with accumulation of the valuable 17β-reduced C19-steroids: testosterone and its ∆1-analogue. Optimization of bioconversion conditions for phytosterols at a high concentration (20 g/L) using a transgenic M. neoaurum-based strain, including the selection of solubilizing agents and optimization of inducer and glucose supplementation regimes, led to an increase in testosterone yield from 5.3  to 6.9 g/L.