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Abstract <p>Moderately halophilic bacteria producing polyhydroxyalkanoates (PHAs), biodegradable polyesters, were isolated from salt, peloid, and coastal soil samples from Elton and Botkul salt lakes using enrichment cultures and subsequent taxonomic analysis. Phenotypic characteristics and phylogenetic analysis showed that all isolates belonged to the genus <i>Bacillus</i>. <i>Bacillus</i> sp. strain B421, when cultivated on a modified Segal−Gibbson medium supplemented with sucrose, synthesized the highest amount of PHA in the form of polyhydroxybutyrate, which was confirmed by FTIR and <sup>1</sup>H NMR spectroscopy data.</p>

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Moderately Halophilic Bacillus sp. Strains as Polyhydroxyalkanoates Producers

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • N. S. Velichko,
  • V. S. Grinev,
  • E. N. Sigida,
  • S. A. Konnova,
  • Yu. P. Fedonenko

摘要

Abstract

Moderately halophilic bacteria producing polyhydroxyalkanoates (PHAs), biodegradable polyesters, were isolated from salt, peloid, and coastal soil samples from Elton and Botkul salt lakes using enrichment cultures and subsequent taxonomic analysis. Phenotypic characteristics and phylogenetic analysis showed that all isolates belonged to the genus Bacillus. Bacillus sp. strain B421, when cultivated on a modified Segal−Gibbson medium supplemented with sucrose, synthesized the highest amount of PHA in the form of polyhydroxybutyrate, which was confirmed by FTIR and 1H NMR spectroscopy data.