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Abstract <p><i>Saccharomyces boulardii</i> (<i>S. boulardii</i>) is a promising probiotic microorganism with diverse health benefits. In the present study, 1 × 10<sup>6</sup> cells of this organism were inoculated in PDB and incubated at 30°C for 24 h in a rotatory shaker (120 rpm) to ensure optimal temperature and metabolite production. Secondary metabolite extraction was carried out, followed by nLC-MS analysis to identify the bioactive compounds. The files generated were analyzed using Compound Discoverer (CD) 3.3, and a ChemSpider database search was conducted to annotate the metabolites. The dataset was matched using the PubChem identifier utility. A total of 331 metabolites were identified. The metabolite spectrum analysis revealed that these bioactive metabolites possessed antimicrobial, antioxidant, anticancer, and anti-inflammatory activities. The metabolites were functionally characterized using existing literature; 25, 10, 5, and 4 of the bioactive compounds exhibited antimicrobial, anticancerous, antioxidant, and anti-inflammatory activity, respectively. This metabolomic study supports the need for further exploration of <i>S. boulardii</i> metabolites, as those with currently unknown activities may also possess valuable pharmacological properties.</p>

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Untargeted Intracellular Metabolomic Profiling of Saccharomyces boulardii Using LC–MS

  • 期刊论文

  • 发表日期：

    2026年7月17日

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  • P. Gautam,
  • Saurav,
  • J. Shankar

摘要

Abstract

Saccharomyces boulardii (S. boulardii) is a promising probiotic microorganism with diverse health benefits. In the present study, 1 × 106 cells of this organism were inoculated in PDB and incubated at 30°C for 24 h in a rotatory shaker (120 rpm) to ensure optimal temperature and metabolite production. Secondary metabolite extraction was carried out, followed by nLC-MS analysis to identify the bioactive compounds. The files generated were analyzed using Compound Discoverer (CD) 3.3, and a ChemSpider database search was conducted to annotate the metabolites. The dataset was matched using the PubChem identifier utility. A total of 331 metabolites were identified. The metabolite spectrum analysis revealed that these bioactive metabolites possessed antimicrobial, antioxidant, anticancer, and anti-inflammatory activities. The metabolites were functionally characterized using existing literature; 25, 10, 5, and 4 of the bioactive compounds exhibited antimicrobial, anticancerous, antioxidant, and anti-inflammatory activity, respectively. This metabolomic study supports the need for further exploration of S. boulardii metabolites, as those with currently unknown activities may also possess valuable pharmacological properties.