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Abstract <p><i>Parvivirga hydrogeniphila</i>, strain Es71-Z0120&#xa0;is a thermophilic bacterium capable of anaerobic growth within the ranges of 25–70°C (optimum at 47–60°C) and pH 6.0–8.5 (optimum at 6.8–7.2) and using H<sub>2</sub> or formate as electron donors and Fe(III) as an acceptor. Genomic analysis revealed the genes of the serine branch of the reductive glycine pathway, while glycine reductase genes were not found. Proteomic studies showed enhanced expression of other key enzymes of this pathway: formate dehydrogenase, methyltetrahydrofolate cyclohydrolase, the glycine cleavage system, and serine hydroxymethyl transferase. The rate of autotrophic carbon assimilation was 0.357 fmol C/(cell day), which was comparable to the values for the known autotrophic microorganisms. These results indicate autotrophic CO<sub>2</sub> assimilation in&#xa0;<i>P.&#xa0;hydrogeniphila</i>&#xa0;, with the serine variant of the reductive glycine pathway being the most probable one. They contribute to our understanding of the metabolic diversity of anaerobic microorganisms and broaden our knowledge of the distribution of various carbon assimilation mechanisms in the microorganisms belonging to the newly desrcibed phylogenetic groups.</p>

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The Serine Variant of the Redox Glycine CO2 Assimilation Pathway in an Anaerobic Thermophile Parvivirga hydrogeniphila

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  • 发表日期：

    2025年11月17日

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  • N. A. Chernyh,
  • I. I. Rusanov,
  • V. A. Pikhtereva

摘要

Abstract

Parvivirga hydrogeniphila, strain Es71-Z0120 is a thermophilic bacterium capable of anaerobic growth within the ranges of 25–70°C (optimum at 47–60°C) and pH 6.0–8.5 (optimum at 6.8–7.2) and using H2 or formate as electron donors and Fe(III) as an acceptor. Genomic analysis revealed the genes of the serine branch of the reductive glycine pathway, while glycine reductase genes were not found. Proteomic studies showed enhanced expression of other key enzymes of this pathway: formate dehydrogenase, methyltetrahydrofolate cyclohydrolase, the glycine cleavage system, and serine hydroxymethyl transferase. The rate of autotrophic carbon assimilation was 0.357 fmol C/(cell day), which was comparable to the values for the known autotrophic microorganisms. These results indicate autotrophic CO2 assimilation in P. hydrogeniphila , with the serine variant of the reductive glycine pathway being the most probable one. They contribute to our understanding of the metabolic diversity of anaerobic microorganisms and broaden our knowledge of the distribution of various carbon assimilation mechanisms in the microorganisms belonging to the newly desrcibed phylogenetic groups.