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Abstract <p>Ability of the haloalkaliphilic anaerobic bacterium <i>Alkaliphilus peptidifermentans</i> Z-7036<sup>T</sup> to use betaine as an electron acceptor in redox reactions was studied. A previously unknown molar stoichiometric ratio of 1 : 2 was shown for growth on serine/threonine with betaine. To explain this phenomenon, a pathway for the degradation of these amino acids was proposed, and the sites of coupling with the betaine reductase complex were determined. Activities of the key enzymes of threonine and serine metabolism were measured. For the first time, the Stickland reaction with betaine was demonstrated for proline and ornithine, and its realization was substantiated. The biochemical mechanisms providing for betaine reduction during growth on ethanolamine were revealed. Genome annotation data were used to construct the schemes for the metabolic pathways of threonine, serine, proline, glutamate, arginine, ornithine, and ethanolamine degradation. For all substrates, metabolic products were revealed and stoichiometric ratios with betaine and the products were obtained.</p>

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Patterns of Betaine Reduction by a Haloalkaliphilic Bacterium Alkaliphilus peptidifermentans Grown on Amino Acids and Ethanolamine

  • 期刊论文

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  • 发表日期：

    2025年11月17日

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  • Yu. V. Boltyanskaya,
  • E. N. Detkova,
  • I. Yu. Oshkin,
  • S. N. Parshina,
  • N. V. Pimenov,
  • V. V. Kevbrin

摘要

Abstract

Ability of the haloalkaliphilic anaerobic bacterium Alkaliphilus peptidifermentans Z-7036T to use betaine as an electron acceptor in redox reactions was studied. A previously unknown molar stoichiometric ratio of 1 : 2 was shown for growth on serine/threonine with betaine. To explain this phenomenon, a pathway for the degradation of these amino acids was proposed, and the sites of coupling with the betaine reductase complex were determined. Activities of the key enzymes of threonine and serine metabolism were measured. For the first time, the Stickland reaction with betaine was demonstrated for proline and ornithine, and its realization was substantiated. The biochemical mechanisms providing for betaine reduction during growth on ethanolamine were revealed. Genome annotation data were used to construct the schemes for the metabolic pathways of threonine, serine, proline, glutamate, arginine, ornithine, and ethanolamine degradation. For all substrates, metabolic products were revealed and stoichiometric ratios with betaine and the products were obtained.