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Abstract <p>The effect of nitrogen and phosphorus stress on the growth and fatty acid profile was studied in the eustigmatophycean alga <i>Vischeria calaminaris</i> (Trzcinska et Pawlik-Skowronska) Kryvenda, Rybalka, Wolf et Friedl. Strain MZ–E6 isolated from soil of a black locust grove is the first finding of <i>Vischeria calaminaris</i> in European Russia. Its taxonomic identification involved morphological analysis and phylogenetic reconstruction based on 18S rRNA gene sequences. Experimental cultures were grown in BBM medium with step changes in the concentrations of nitrogen (from 0 to 0.6 g/L) and phosphorus (from 0 to 0.3 g/L). Cultivation of strain MZ–E6 for 20 days resulted in biomass accumulation ranging from 3.36 to 4.48 g/L dry weight, with the maximum yield achieved under conditions of moderate limitation by nitrogen (0.15 g/L). In all culture variants, the dominant fatty acids were palmitic, palmitoleic, oleic, and eicosapentaenoic: they accounted for 84.67–87.02% of the total fatty acid content. It was found that phosphorus stress caused more pronounced changes in the fatty acid composition than nitrogen stress. It was shown that an efficient strategy is culturing strain <i>Vischeria calaminaris</i> MZ–E6 in BBM containing 0.2 g/L nitrogen and 0.11 g/L phosphorus: this allows achieving substantial biomass yield (4.19 g/L) with a high content of saturated and polyunsaturated fatty acids (over 66% in total).</p>

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Effect of Nitrogen and Phosphorus Stress on Growth and Fatty Acid Composition in Microalga Vischeria calaminaris (Eustigmatophyceae)

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Z. V. Krivova,
  • S. Y. Maltseva,
  • D. D. Minnullin,
  • M. S. Kulikovskiy,
  • Y. I. Maltsev

摘要

Abstract

The effect of nitrogen and phosphorus stress on the growth and fatty acid profile was studied in the eustigmatophycean alga Vischeria calaminaris (Trzcinska et Pawlik-Skowronska) Kryvenda, Rybalka, Wolf et Friedl. Strain MZ–E6 isolated from soil of a black locust grove is the first finding of Vischeria calaminaris in European Russia. Its taxonomic identification involved morphological analysis and phylogenetic reconstruction based on 18S rRNA gene sequences. Experimental cultures were grown in BBM medium with step changes in the concentrations of nitrogen (from 0 to 0.6 g/L) and phosphorus (from 0 to 0.3 g/L). Cultivation of strain MZ–E6 for 20 days resulted in biomass accumulation ranging from 3.36 to 4.48 g/L dry weight, with the maximum yield achieved under conditions of moderate limitation by nitrogen (0.15 g/L). In all culture variants, the dominant fatty acids were palmitic, palmitoleic, oleic, and eicosapentaenoic: they accounted for 84.67–87.02% of the total fatty acid content. It was found that phosphorus stress caused more pronounced changes in the fatty acid composition than nitrogen stress. It was shown that an efficient strategy is culturing strain Vischeria calaminaris MZ–E6 in BBM containing 0.2 g/L nitrogen and 0.11 g/L phosphorus: this allows achieving substantial biomass yield (4.19 g/L) with a high content of saturated and polyunsaturated fatty acids (over 66% in total).