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Abstract <p>The <i>TPS1</i> and <i>TPS2</i> genes encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase play an important role in protecting yeast and fungal cells from temperature stress, dehydration, and oxidants, since resistance to these stresses requires accumulation of trehalose. In this work, it was shown that <i>Saccharomyces cerevisiae</i> strains with knockout mutations in the <i>TPS1</i> and <i>TPS2</i> genes have greater resistance to heavy metal ions and alkaline stress compared to the parental strain. These mutations do not lead to significant changes in the polyphosphate content in the cells, indicating the absence of a direct relationship between phosphorus homeostasis and the ability to accumulate trehalose. In the cells of ∆<i>tps2</i>, the change in localization of the Bgl2p cell wall protein was observed: this protein appeared to be intracellular. It is assumed that the resistance of knockout strains to heavy metal ions and alkali is associated with additional pleiotropic effects of these mutations.</p>

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Pleiotrophic Effects of Knockout Mutations in the TPS1 and TPS2 Genes Encoding Trehalose Biosynthesis Enzymes of Saccharomyces cerevisiae

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • E. V. Kulakovskaya,
  • V. I. Torgov,
  • N. E. Nifantiev,
  • V. V. Rekstina,
  • T. S. Kalebina

摘要

Abstract

The TPS1 and TPS2 genes encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase play an important role in protecting yeast and fungal cells from temperature stress, dehydration, and oxidants, since resistance to these stresses requires accumulation of trehalose. In this work, it was shown that Saccharomyces cerevisiae strains with knockout mutations in the TPS1 and TPS2 genes have greater resistance to heavy metal ions and alkaline stress compared to the parental strain. These mutations do not lead to significant changes in the polyphosphate content in the cells, indicating the absence of a direct relationship between phosphorus homeostasis and the ability to accumulate trehalose. In the cells of ∆tps2, the change in localization of the Bgl2p cell wall protein was observed: this protein appeared to be intracellular. It is assumed that the resistance of knockout strains to heavy metal ions and alkali is associated with additional pleiotropic effects of these mutations.